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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3628-3635
DOI: https://doi.org/10.20546/ijcmas.2017.608.437


Evaluation of Substrates for Mass Production of Trichoderma harzianum and its Compatibility with Chlorpyrifos + Cypermethrin
N.S. Siddhartha1, K.V. Amara1, K.A. Ramya Mol2, K.A. Saju2*,K.N. Harsha2, P. Sharanappa1 and K. Pradip Kumar2
1Department of Botany, Post Graduate Centre, University of Mysore,Hemagangothri 573220, Karnataka, India
2Indian Cardamom Research Institute, Regional Station, Spices Board,Sakleshpur 573134, Karnataka, India
*Corresponding author
Abstract:

Coir pith compost, rice husk and sugarcane bagasse with and without 5% Jaggery solution as nutritional supplement was evaluated for the mass production of Trichoderma harzianum in comparison with coffee husk (parchment). On incubation up to 60 days, maximum number of propagules were recorded in coir pith compost (43 × 1010), coir pith compost + 5% jaggery (32.33 × 1010) and sugarcane bagasse (21 × 1010) followed by rice husk (38 × 1010) and rice husk + 5% jaggery (23.33 × 1010). Coir pith compost and sugarcane bagasse recorded more population than other substrates and hence could be used in mass production. In another experiment, the compatibility of T. harzianum and insecticide combination, Chlorpyrifos 50% + Cypermethrin 5% was studied by poisoned food technique on PDA medium at concentrations of 55, 83, 110, 138, 165, 193 and 220 ppm. In the first set, the insecticide was added to the autoclaved medium (hence not heated) and in the second set the insecticide was autoclaved along with the medium. In both the cases, the colony growth was very slow initially for 5 days, however continued to grow as evident from the measurements on 10th and 15th day. Thus the insecticide was partially compatible with T. harzianum in in-vitro studies at all the concentration levels. Later, T. harzianum and Chlorpyrifos + Cypermethrin were incorporated into soil taken in containers so as to get the above insecticide concentrations and studied the compatibility by monitoring the population by serial dilution plate technique. After 30 days, it was found that the propagules in all the insecticide concentrations were on par with each other. Hence it is implied that the insecticide did not affect the population of T. harzianum in in-vivo conditions and found compatible for field application.


Keywords: Chlorpyrifos, compatibility, Cypermethrin,Mass production, Substrates and Trichoderma.
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How to cite this article:

Siddhartha, N.S., K.V. Amara, K.A. Ramya Mol, K.A. Saju, K.N. Harsha,  P. Sharanappa and Pradip Kumar, K. 2017. Evaluation of Substrates for Mass Production of Trichoderma harzianum and its Compatibility with Chlorpyrifos + Cypermethrin.Int.J.Curr.Microbiol.App.Sci. 6(8): 3628-3635. doi: https://doi.org/10.20546/ijcmas.2017.608.437