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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 659-666
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.075


Isolation and Molecular Characterization of Cry Gene for Bacillus thuringiensis Isolated from Soil of Gaza Strip
Azme Dagga1, Mohamed Abdel Aziz2, Abed Alraoof Al Amnama3, Mervat Al-Sharif4 and Mahmoud El Hindi5*
1Microbiology, ERRC, Islamic University of Gaza, Palestine
2Microbiology, Botany Department, Faculty of Science, Suez Canal University Ismailia, Egypt
3Microbiology, Department of Medical and laboratories sciences, Islamic University of Gaza, Palestine
4Microbiology, Faculty of Education, Alexandaria University, Egypt
5Microbiology Lab, Department of Biology and Biotechnology, Islamic University of Gaza, Palestine

*Corresponding author
Abstract:

Gaza strip is a narrow piece of land lying in the coast of the Mediterranean Sea. Its position on the cross roads from Africa to Asia made it a target for occupiers and conquerors over the centuries. The Gaza Strip is situated in the south part of historical Palestine and southeast of the mediterranean. Ten soil samples were collected from different agricultural and non-agricultural soils from different locations in Gaza strip, and all samples of Bacillus thuringiensis strains were isolated from soil. B. thuringiensis is a gram positive and spore forming bacterium. The most of the natural habitat for this bacterium is soil and it is capable of producing the diversified varieties of crystal proteins (cry proteins) with insecticide property. Polymerase Chain reaction (PCR) is a molecular tool widely used to characterize the insecticidal bacterium B. thuringiensis. This technique can be used to amplify specific DNA fragments and thus to determine the presence or absence of a target gene. The molecular identification of Bt cells through the PCR analysis of the delta-endotoxins genes coupled to ribotyping, is an innovative method, that has enabled the identification of this organism into wetland environments.


Keywords: Bacillus thuringiensis, Insecticide, Polymerase chain reactions (PCR), Crystalline protein, Cry gene, Gaza strip.
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How to cite this article:

Azme Dagga, Mohamed Abdel Aziz, Abed Al'raoof Al Amnama, Mervat Al-Sharif and Mahmoud El Hindi. 2016. Isolation and Molecular Characterization of Cry Gene for Bacillus thuringiensis Isolated from Soil of Gaza Strip. Int.J.Curr.Microbiol.App.Sci. 5(4): 659-666. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.075