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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3521-3530
DOI: https://doi.org/10.20546/ijcmas.2017.608.421


Actinomycetes from Mountains of Hadhramout – Yemen
Ali Mohammed Abdullah Bawazir1, Ahmed Al-Haddad2,Abdullah AL-Mahdi3 and Manjula Shantaram*4
1Department of Microbiology, Mangalore University, Post Graduate Centre,Chikka Aluvara, Kodagu, Karnataka, India
2Department of Medical Microbiology, Faculty of Medicine, Hadhramout University, Yemen
3Biology Department, Microbiology Division, Faculty of Science, Sana’a University, Yemen
4Department of Studies in Biochemistry, Mangalore University, Post Graduate Centre, Chikka Aluvara, Kodagu, Karnataka, India
*Corresponding author
Abstract:

In this study, the soil samples were collected from four different mountain sites of Hadhramout governorate in Yemen to investigate the diversity of actinomycetes. Soil samples were analyzed for physicochemical characteristics (temperature, pH and nature of the soil samples). Site soil color varied from brown to light brown and dark brown, temperature of the locations ranging from 33ºC to 39.1ºC and pH from 7 to 9. Actinomycetes were isolated by serial dilution and plating method on starch casein agar media. Total 56 species were isolated on the bases of colony characteristics on starch casein agar and all the isolates were screened for antibacterial activity by agar disk diffusion method (ADD) and agar well diffusion method (AWD). So, 30% isolates have shown activity against Bacillus subtilis by ADD and 14% isolates by AWD. For Staphylococcus aureus 24% isolates had activity by ADD and 8% isolates had activity by AWD. There were 33% isolates showing activity against Pseudomonas aeruginosa by ADD and 15% isolates by AWD. The activity was low against Escherichia coli, only 24% isolates have shown activity by ADD and 11% isolates have shown activity by AWD. In this study, we noticed MKr123/1 isolate has a strong activity against both Gram positive and Gram negative bacteria so that MKr123/1 isolate was selected and it is identified as Nocardia otitidiscaviarum.


Keywords: Mountain soils, Actinomycetes, Antibiotic, Antibacterial activity, Starch Casein agar, MKr123/1.
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How to cite this article:

Ali Mohammed Abdullah Bawazir, Ahmed Al-Haddad, Abdullah AL-Mahdi and Manjula Shantaram. 2017. Actinomycetes from Mountains of Hadhramout – Yemen.Int.J.Curr.Microbiol.App.Sci. 6(8): 3521-3530. doi: https://doi.org/10.20546/ijcmas.2017.608.421