National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Amylase producing thermophilic bacterial strain was isolated from Jeori hot water spring, Rampur Bushahr of Shimla district of Himachal Pradesh, India and identified as Bacillus thuringiensis J2 using 16S rRNA gene sequencing and deposited in NCBI gene bank vide accession number |KY990713|. Medium components and process parameters were optimized with respect to substrate concentration, carbon sources, nitrogen sources, incubation period, pH and temperature for an enhanced amylase production. Highest amylase activity of 61.35 IU was obtained in apple pomace as a low cost substrate at a temperature of 45°C, pH 9 and incubation period of 72 h with starch and yeast extract as best carbon and nitrogen sources.
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