Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2353-2361
DOI: https://doi.org/10.20546/ijcmas.2017.608.278


RAPD Based Genetic Diversity Analysis in 25 Genotypes of Withania somnifera (L.) Dunal
Surya Chauhan*, Arunabh Joshi and Devendra Jain
Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur 31300, (Rajasthan), India
*Corresponding author
Abstract:

Genetic diversity was evaluated among 25 genotypes of Withania somnifera L. Dunal using RAPD marker. The 15 RAPD primers screened amplified 150 bands among which 141 bands (94%) were polymorphic. The Polymorphic Information Content (PIC) values ranged from 0.162 (OPA-05) to 0.422 (OPA-02) with an average of 0.310. A total of five primers detected in the study produced seven unique bands in seven genotypes Jaccards similarity coefficient values ranged from 0.29 to 0.85 with an average of 0.57. Cluster analysis based on Jaccards similarity coefficient using Un-weighted Pair Group Method with Arithmetic Averages (UPGMA) grouped all the 25 genotypes into two major groups. The cluster I is a major cluster consisting of 20 genotypes whereas cluster II which is minor consists of 5 genotypes. The results show that the level of genetic variation was high among the Ashwagandha genotypes.


Keywords: RAPD, Genetic diversity, 25 genotypes.
Download this article as Download

How to cite this article:

Surya Chauhan, Arunabh Joshi and Devendra Jain. 2017. RAPD Based Genetic Diversity Analysis in 25 Genotypes of Withania somnifera (L.)Int.J.Curr.Microbiol.App.Sci. 6(8): 2353-2361. doi: https://doi.org/10.20546/ijcmas.2017.608.278