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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2343-2351
DOI: https://doi.org/10.20546/ijcmas.2017.608.277


Evaluation of Phenolics and Antioxidant Enzyme Systems for Phytophthora Blight in Resistant and Susceptible Variety of Sesame (Sesamum indicum L.)
D.S. Mori*, U.K. Kandoliya, V.S. Bhatt, S.V. Patel and B.A. Golakiya
Department of Biotechnology, College of agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding author
Abstract:

The present experiment was conducted with an objective to evaluate changes in total phenol and antioxidant enzymes in resistance and susceptible cultivars of sesames against Phytophthora blight. Among the sesame cultivars, root of resistant cultivar G. Til-10 showed the highest amount of total phenol content (0.846 mg.g-1Fr.Wt.), while infected leaf tissue of susceptible cultivar G. Til-4 contained the lowest value(0.418 mg.g-1Fr.Wt.). The activity of ROS scavenging enzymes superoxide dismutase (SOD), Guaiacol peroxidase (GPX), Ascorbate peroxidase (APX) and catalase (CAT) was remained higher in infected root and leaf tissue of the susceptible cultivar G. Til-4 compared to resistant genotypes. The activity of superoxide dismutase was increased an average 1-2 fold in susceptible genotype from pre-infection to infection stage. In case of GPX activity the activity continuously increased in infected tissue from pre-infection to post-infection stage with respect to non-infected tissue. The higher activity was recorded in susceptible cultivar G. Til-4. Same trend of result observed in ascorbate peroxidase activity. In case of polyphenol oxidase, highest activity was noted in infected leaf and root tissue of susceptible (G. Til-4) cultivar. The activity of catalase showed increased in activity of infected tissue compared to non-infected.


Keywords: Sesame, Phytophthora blight, Superoxide dismutase (SOD), Guaiacol peroxidase (GPX), Ascorbate peroxidase (APX), Catalase (CAT).
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How to cite this article:

Mori, D.S., U.K. Kandoliya, V.S. Bhatt, S.V. Patel and Golakiya, B.A. 2017. Evaluation of Phenolics and Antioxidant Enzyme Systems for Phytophthora Blight in Resistant and Susceptible Variety of Sesame (Sesamum Indicum L.).Int.J.Curr.Microbiol.App.Sci. 6(8): 2343-2351. doi: https://doi.org/10.20546/ijcmas.2017.608.277