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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2304-2309
DOI: https://doi.org/10.20546/ijcmas.2017.608.271


Molecular Confirmation of Rhipicephalus haemaphysaloides Infesting Ruminants in Wayanad, Kerala, India
Murikoli Nimisha1, Reghu Ravindran1*, Pradeep R. Kariyappa1, B. Mallappa Amrutha1, Prashant Somalingappa Kurbet1, Karapparambu Gopalan Ajith Kumar1,Anju Varghese1, Chundayil Kalarikkal Deepa1 and Sanis Juliet2
1Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad-673576, Kerala, India
2Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, P.O., Wayanad-673576, Kerala, India
*Corresponding author
Abstract:

Rhipicephalus haemaphysaloides is a prevalent multi host tick species with high vector potential in south India. There are several reports based on morphological identification of the tick species from various parts of the country. However, there were limited attempts for molecular confirmation of this tick species. In the present study, the presence of R. haemaphysaloides in Wayanad, Kerala was confirmed by polymerase chain reaction based on amplification of mitochondrial 16S rRNA gene and V4 region of 18S rRNA gene. Two tick samples, one engorged on a bovine male calf of Pookode, Wayanad and other engorged on a sambar deer in Meppadi were used for the study. Phylogeny based on 16S rRNA and 18S rRNA of R. haemaphysaloides revealed genetic relatedness with Chinese isolates of the tick species.


Keywords: Rhipicephalus haemaphysaloides, PCR, Phylogeny, Wayanad, Kerala.
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How to cite this article:

Murikoli Nimisha, Reghu Ravindran, Pradeep R. Kariyappa, B. Mallappa Amrutha, Prashant Somalingappa Kurbet, Karapparambu Gopalan Ajith Kumar, Anju Varghese, Chundayil Kalarikkal Deepa and Sanis Juliet. 2017. Molecular Confirmation of Rhipicephalus haemaphysaloides Infesting Ruminants in Wayanad, Kerala.Int.J.Curr.Microbiol.App.Sci. 6(8): 2304-2309. doi: https://doi.org/10.20546/ijcmas.2017.608.271