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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2280-2286
DOI: https://doi.org/10.20546/ijcmas.2017.608.267


Study of Production of Green Conjugates of Silver Nanoparticles for Determination of Antimicrobial Potential against Biofilm Producing Staphylococcus aureus
Jaya Bharti1*and Abhishek Mathur2
1Department of Microbiology, Mewar University, Chittorgarh, Rajasthan, India
2R&D cum New product Development, EBEC-NCFT, New Delhi, India
*Corresponding author
Abstract:

In recent era, Nanotechnology is a burning field for the researchers. Nanotechnology deals with the Nanoparticles having a size of 1-100 nm in one dimension used significantly concerning medical chemistry, atomic physics, and all other known fields. Nanoparticles are used immensely due to its small size, orientation, physical properties, which are reportedly shown to change the performance of any other material which is in contact with these tiny particles. These particles can be prepared easily by different chemical, physical, and biological approaches. But the biological approach is the most emerging approach of preparation, because, this method is easier than the other methods, eco-friendly and less time consuming.  Staphylococcus aureus is a leading cause of nosocomial infections and the etiologic agent of a wide range of diseases associated with significant morbidity and mortality. Some of the diseases mediated by this species include endocarditis, osteomyelitis, toxic shock syndrome, food poisoning, and skin infections. Biofilms are well organized and complex aggregate of microorganisms, surrounded by a protective matrix of exo-polysaccharides and can adhere to each other on various surfaces. Because of increase in the drug resistance and the side effect of over use of antibiotic, it is necessary to find a suitable agent to reduce the growth of microorganism. Biofilm are known as a significant problem because biofilm formation protects pathogenic bacteria against antibiotics and is one of the main causes of development of chronic infections. Thus biofilm is the main cause of drug resistance in microbial strains. These strains cause pathogenicity to a maximum extent and thus cause resistance against the conventional antibiotics/drugs. The nanoparticles were synthesized by the concept of green synthesis by using the aqueous solution of Azadirachta indica leaf extract and silver nitrate. Silver was of a particular interest for this process due to its evocative physical and chemical properties. Staphylococcus aureus strains were isolated and screened for biofilm production. A fixed ratio of plant extract to metal ion was prepared and the color change was observed which proved the formation of nanoparticles. These nanoparticles were characterized by UV-Vis Spectrophotometer and SEM. The nanoparticles were found have the size ranges from 160-180 nm. These fused Ag-Azadirachta indica* (Ag-AI*) nanoparticles were screened against biofilm producing strains of S. aureus. The results showed promising antimicrobial activity against these biofilm producers.


Keywords: Green synthesis, Silver fused Azadirachta indica nanoparticles, Biofilm, S. aureus, Antimicrobial activity.
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How to cite this article:

Jaya Bharti and Abhishek Mathur. 2017. Study of Production of Green Conjugates of Silver Nanoparticles for Determination of Antimicrobial Potential against Biofilm Producing Staphylococcus aureus.Int.J.Curr.Microbiol.App.Sci. 6(8): 2280-2286. doi: https://doi.org/10.20546/ijcmas.2017.608.267