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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2267-2279
DOI: https://doi.org/10.20546/ijcmas.2017.608.266


Herbal Immersion Oil for Microscopic Identification of Malaria Parasites
Ayushi Rastogi1,3, V.K. Dua1*, V.K. Varshney2, N.C. Gupta1 and Sumit Kumar3
1National Institute of Malaria Research, Field Station, BHEL, Haridwar, India
2Chemistry Department, Forest Research Institute, Dehradun, India
3Shri Venketeshwara University, Gajraula, Amroha, India
*Corresponding author
Abstract:

Oils from thirty-two plants were extracted using solvent extraction/steam distillation methods. All oils along with 11 blended oil mixtures were   tested   for their immersion oil properties for the detection of malaria parasites. The physicochemical properties required for an immersion oil including density, viscosity, refractive index and acid value of five oils namely as Ocimum basilicum, Pogostemon cablin, Papaver somniferum Ricinus communis and Valeriana jatamansi and   blended mixture 6 were determined. Stability   of above stated plant oils and blended mixture 6, with time showed that all were stable under the observation period while non-dryness of these oils and blended mixture 6, represented very good criteria for the non-drying less than 1% variation in weight. GC-MS analysis of mixture 6 clearly identified compounds namely alpha copane, trans-Caryophyllene Linalool L, Estragole, hexadecanoic acid phenyl methyl ester and 9, 12-Octadecadienoic acid and besides other minor components peaks.  No peak of castor oil was detected due to lack of sample character and detection. Summary of in-house validation of blended mixture 6 classified as very good immersion oil (range 67-81%) and good (19-33%) for the examination of malaria parasites while the results of external validation clearly revealed that the developed plant oil mixture 6 possessed very good property as immersion oil for the examination of malaria parasites.


Keywords: Malaria parasites, Immersion oil, Plant oil mixture.
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How to cite this article:

Ayushi Rastogi, V.K. Dua, V.K. Varshney, N.C. Gupta and Sumit Kumar. 2017. Herbal Immersion Oil for Microscopic Identification of Malaria Parasites.Int.J.Curr.Microbiol.App.Sci. 6(8): 2267-2279. doi: https://doi.org/10.20546/ijcmas.2017.608.266