National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Oils from thirty-two plants were extracted using solvent extraction/steam distillation methods. All oils along with 11 blended oil mixtures were tested for their immersion oil properties for the detection of malaria parasites. The physicochemical properties required for an immersion oil including density, viscosity, refractive index and acid value of five oils namely as Ocimum basilicum, Pogostemon cablin, Papaver somniferum Ricinus communis and Valeriana jatamansi and blended mixture 6 were determined. Stability of above stated plant oils and blended mixture 6, with time showed that all were stable under the observation period while non-dryness of these oils and blended mixture 6, represented very good criteria for the non-drying less than 1% variation in weight. GC-MS analysis of mixture 6 clearly identified compounds namely alpha copane, trans-Caryophyllene Linalool L, Estragole, hexadecanoic acid phenyl methyl ester and 9, 12-Octadecadienoic acid and besides other minor components peaks. No peak of castor oil was detected due to lack of sample character and detection. Summary of in-house validation of blended mixture 6 classified as very good immersion oil (range 67-81%) and good (19-33%) for the examination of malaria parasites while the results of external validation clearly revealed that the developed plant oil mixture 6 possessed very good property as immersion oil for the examination of malaria parasites.
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