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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2241-2248
DOI: https://doi.org/10.20546/ijcmas.2017.608.263


Assessment of Genetic Diversity for Storage Seed Protein in a Set of Released Varieties and their Diallel Set of Crosses in Indian Mustard [Brassica juncea (L.) Czern. & Coss.] Using SDS PAGE
Kuldeep Jangid*, K. Ram Krishna, Naresh Parashar, Bhawana Bhatt,Arpit Gaur and Vinod Kumar
Department of Plant Breeding and Genetics, S.K.N. College of Agriculture, Jobner
Sri Karan Narendra Agriculture University, Jobner, Jaipur – 303329, Rajasthan, India
*Corresponding author
Abstract:

Brassica species are major oilseed crops which are an increasingly important part of the human diet worldwide. In present investigation 11 varieties and their 55 F1’s were characterized for storage seed protein profiling using sodium dodecyl sulphate – polyacryl amide gel electrophoresis (SDS - PAGE). The electrophoresis of the proteins revealed protein bands in the range of 80kD to less than 14 kD molecular weight (MW). Out of 15 bands 14 bands were more or less monomorphic and only single band was polymorphic. Gels were scored for the presence (1) and absence (0) for polymorphic band. The similarity coefficient among these genotypes ranged from 1 to 0.25. A large number of pairs assumed value of 1.0 (61.6 %) followed by 0.8 (35.4%) about 3% cases showed similarity coefficient of less than 0.8. 


Keywords: Brassica, SDS-PAGE, Cluster analysis, UPGMA.
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How to cite this article:

Kuldeep Jangid, K. Ram Krishna, Naresh Parashar, Bhawana Bhatt, Arpit Gaur and Vinod Kumar. 2017. Assessment of Genetic Diversity for Storage Seed Protein in a Set of Released Varieties and their Diallel Set of Crosses in Indian Mustard [Brassica juncea (L.) Czern. & Coss.] Using SDS PAGE.Int.J.Curr.Microbiol.App.Sci. 6(8): 2241-2248. doi: https://doi.org/10.20546/ijcmas.2017.608.263