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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2179-2190
DOI: https://doi.org/10.20546/ijcmas.2017.608.258


Assessment the Effects of Low Power Laser Light on Some Virulent Factors of Klebsiella pneumoniae Isolated from Respiratory Tract Infections
Diana Fadhil Al-saadi1*, Mouruj A. Al-Aubydi1 and Layla M. Al-Ameri2
1Biotech. Dept. University of Baghdad/College of science, Iraq
2University of Baghdad/ Institute of laser for postgraduate studies, Iraq
*Corresponding author
Abstract:

One of the most opportunistic and nosocomial infections causative agent is K. pneumoniae, its tendency to be chronic and often display multidrug-resistance phenotypes that are commonly caused by the presence of Extended-spectrum β-lactamases or carbapenemases, making it difficult to choose appropriate antibiotics for treatment. Opportunistic K. pneumoniae mostly affects those with compromised immune systems or who are weakened by other infections. Its colonization can be made further infection in the human especially in the urinary tract, and respiratory tract. Thus this study was a goal to assess the effect of low power laser light on the virulence factors of Klebsiella pneumoniae. The results showed 17.9% were characterized as Klebsiella pneumoniae from 151 cases of respiratory tract infections. Antimicrobial sensitivity test showed elevation in the resistance of these isolates for most usable antimicrobial agents, the most resistant isolate was selected. Another selection was done depending on the type of laser dye, the result of using methylene blue dye at 650 nm. Showed the numbers of the colony were varied according to the conc. of dye, they were direct decreases due to the decreasing of dye conc. with time. Meanwhile, it became smaller with rough texture when cultured on MaCconkey agar, and microscopically the bacterial capsules showed slight thickness around the bacteria in compared with the control isolate. As well as there was changing in the degree of glucose fermentation. On the other hand, the antimicrobial sensitivity results showed, the exposed isolate was changed the sensitivity condition to resistance for both of Ciprofloxacin (CIP) and Tobramycin (TN). While using another laser dyes (Rhodamine110 and Anthocyanin) at 532 nm. There was variation in the colony size and texture. Meanwhile microscopically, the bacterial capsules elucidated slight thickness around the bacteria in compared with the control isolate. Inaddition to the changing in some biochemical tests, as well as the antimicrobial sensitivity results showed heterogeneities toward several antimicrobial agents. On the other hand, the results of biofilm formation showed a negative result for all tests.


Keywords: Klebsiella pneumoniae, Low power laser light, Methylene blue, Rhodamine dyes and Virulent factors.
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How to cite this article:

Diana Fadhil Al-saadi, Mouruj A. Al-Aubydi and Layla M. Al-Ameri. 2017. Assessment the Effects of Low Power Laser Light on Some Virulent Factors of Klebsiella pneumoniae Isolated from Respiratory Tract Infections.Int.J.Curr.Microbiol.App.Sci. 6(8): 2179-2190. doi: https://doi.org/10.20546/ijcmas.2017.608.258