Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2171-2178
DOI: https://doi.org/10.20546/ijcmas.2017.608.257


Characterization of Genetic Diversity of Rice Genotypes Showing Resistance to Rice Leaf Folder, Cnapholocrocis medinalis Using SSR Markers
Kamakshi1*, P. Rajasekhar2, G. Ramachandra Rao3, P. Anil Kumar3 and P.N. Harathi
1Regional Agricultural Research Station, Nandyal, Kurnool (Dist), A.P.-518503, India
2Agricultural Research Station, Nellore, SPSR Nellore (Dist), A.P. -524301, India
3Agricultural College, Bapatla, Guntur (Dist.), A.P. – 522101, India
4Agricultural Research Station, Nellore, SPSR Nellore (Dist), A.P. -524301, India
*Corresponding author
Abstract:

Twenty rice genotypes which recorded resistant and moderately susceptible reaction against rice leaf folder were characterized by using SSR markers. Out of eleven primers of SSR markers tested, seven primers were found to be polymorphic in nature. A total of 123 amplified bands were seen with 78 % polymorphism with the primers RM 3691, RM 600, RM 14 and RM 228. And it was also observed that all the bands were in the range of 100 bp to 1000bp.  A clear variation in the banding pattern was observed among the resistant and susceptible genotypes with the primer RM 3691. The Jaccard’s similarity values ranged from 0-89 % indicating the presence of wide range of genetic diversity. The dendrogram showed variable degree of relationship among the genotypes. The resistant and moderately susceptible genotypes were grouped into two different clusters indicating that they were significantly different at molecular level also. In cluster-I, INRC-3021 and W1263 were very closely related and in cluster-II, NLR 145 and Sri Satya were closely related with the similarity index of 86 and 89 %, respectively.


Keywords: Rice leaffolder, Cnapholocrocis medinalis, SSR Markers, Resistance.
Download this article as Download

How to cite this article:

Kamakshi, N., P. Rajasekhar, G. Ramachandra Rao, P. Anil Kumar and Harathi, P.N. 2017. Characterization of Genetic Diversity of Rice Genotypes Showing Resistance to Rice Leaf Folder, Cnapholocrocis medinalis Using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 6(8): 2171-2178. doi: https://doi.org/10.20546/ijcmas.2017.608.257