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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 1690-1702
DOI: https://doi.org/10.20546/ijcmas.2017.608.203


Thidiazuron Induced Direct Shoot Organogenesis in Stevia rebaudiana and Assessment of Clonal Fidelity of Regenerated Plants by RAPD and ISSR
Manvendra Singh*, Vinod Saharan, Deepak Rajpurohit, Yadunandan Sen,Arunabh Joshi and Ajay Sharma
Department of Molecular Biology and Biotechnology, RCA, Maharana Pratap University of Agriculture and Technology Udaipur-313001, Rajasthan, India
*Corresponding author
Abstract:

Stevia rebaudiana is a herbaceous perennial plant of Asteraceae family and an excellent substitute of sugar. Increasing demand of quality panting material has emphasized the need of rapid and mass multiplication through plant tissue culture technology. Therefore, high frequency mass multiplication of Stevia was established using in vivo and in vitro nodal explants. MS basal medium supplemented with 2.0 mg/l TDZ induced maximum shoot induction (68.3%) from in vivo nodal segments and 2.5 mg/l TDZ showed 100% shoot induction from in vitro nodal explants. The highest shoot multiplication (7.33 shoots/explant) was found on MS medium supplemented with 1.0 mg/l BAP+ 0.5 mg/l Kin. Root induction was performed on MS basal medium supplemented with different concentration of IAA, IBA and NAA. Quality root formation with respect to root length and root number per microshoots was observed on hormone free MS basal medium. Rooted plantlets were hardened in vermiculite and cocopeat in the ratio of 1:1 for 3 weeks and successfully acclimatized in field condition. Assessment of clonal fidelity of in vitro raised plantlets was performed by RAPD and ISSR molecular analysis which revealed that all the regenerated plants of stevia through tissue culture were true-to-type compared to mother plants. The protocol developed in the present study could be used for production of approximately 2400 true-to-type plants in 12 week from single microshoot from 4 subcultures.


Keywords: Clonal fidelity, Molecular marker, Stevia, Tissue culture, TDZ (Thidiazuron).
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How to cite this article:

Manvendra Singh, Vinod Saharan, Deepak Rajpurohit, Yadunandan Sen, Arunabh Joshi and Ajay Sharma. 2017. Thidiazuron Induced Direct Shoot Organogenesis in Stevia rebaudiana and Assessment of Clonal Fidelity of Regenerated Plants by RAPD and ISSR.Int.J.Curr.Microbiol.App.Sci. 6(8): 1690-1702. doi: https://doi.org/10.20546/ijcmas.2017.608.203