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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3338-3348
DOI: https://doi.org/10.20546/ijcmas.2017.608.398


Genetic Characterization of Locally Cultivated Taro Germplasm from Eleven District of Nagaland, India
Thejazhanuo Lulu Mezhii1, Sapu Changkija1, A. Pattanayak2, H.P. Chaturvedi1,S.  Vimala Devi3 and Pravas R. Kole4*
1Department of Genetics and Plant Breeding, Nagaland University, SASRD,Medziphema- 797106, India
2ICAR RC for NEHR, Umiam, Meghalaya- 793103, India
3Division of Germplasm Conservation, National Bureau of Plant Genetic Resources,New Delhi, India
4Protection of Plant Variety and Farmer Right Act, NASC Complex,DPS Marg, New Delhi, India
*Corresponding author
Abstract:

Colocasia or taro (Colocasia esculanta L.) is one of the important tuber crops, which has been grown by farmers in rain-fed ecosystem and considered as an important food crop after rice in Nagaland. The characterization of taro from 11 districts of Nagaland, Northeast India has been analyzed using microsatellite markers. Twenty eight microsatellite markers were used to analyze 50 accessions of taro collected from 11 district of Nagaland. A total of 53 alleles were amplified at an average of 1.89 alleles per locus. Both altered alleles and null alleles were observed. The number of alleles ranged from one to four. The overall size of amplified products ranged from 117bp to 685bp. Dendrogram based on UPGMA analysis separated the accessions into five clusters. Four definite clusters were identified at a level of 35% similarity among the individuals. A three dimensional plot prepared from the principal component analysis of fifty-three alleles with PIC values ranging from 0.41 - 0.93 indicating high specificity and discriminatory power of the markers. Analysis of molecular variance (AMOVA) revealed most variation among individuals within population at 100%. It appears that high within population variation is a characteristic of colocasia. The present study showed that the germplasm of Nagaland was diverse but somewhat uniformly distributed across the state. Further exploration and collection of colocasia germplasm is required.


Keywords: Taro, Colocasia esculanta, Microsatellite, Molecular marker
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How to cite this article:

Thejazhanuo Lulu Mezhii, Sapu Changkija, A. Pattanayak, H.P. Chaturvedi, S. Vimala Devi and Pravas R. Kole. 2017. Genetic Characterization of Locally Cultivated Taro Germplasm from Eleven District of NagalandInt.J.Curr.Microbiol.App.Sci. 6(8): 3338-3348. doi: https://doi.org/10.20546/ijcmas.2017.608.398