National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study intended to characterize fifteen elite Indian rice varieties with the help of molecular markers, to develop molecular ID and to analyze the genetic purity of four selected varieties viz., MTU 1010, MTU 7029, BPT 5204 and Improved Samba Mashuri in different seed lots through Grow-Out Test (GOT) and molecular marker-based assays. The results of both assays were also compared. A total of 48 SSR markers distributed across the twelve rice chromosomes were employed producing 69 alleles which amplified among the fifteen rice genotypes. The Polymorphic Information Content (PIC) value ranged from 0.03 to 0.64. The primers RM24888 with an allele size of 200 bp uniquely identified the variety MTU 1010 from others. The marker combination of RM206 and ESSR12-20.2 with an allele size of 450 bp and 170 bp respectively, identified the variety MTU 7029 uniquely. The marker combination of RM10001 and RM206 with allele size of 480 bp and 450 bp respectively, identified the variety BPT 5204 uniquely. The marker combination of JGT06-6.81 and RM22266 with allele size of 450 bp and 200 bp respectively, identified the variety Improved Samba Mashuri uniquely. Thus, these marker or marker combination served as molecular IDs. On assessing foundation and certified seed lots (truthfully labeled in case of Improved Samba Mashuri) for genetic purity through GOT and molecular markers, impurity percentage detected through molecular markers was 0.75 to 2.75%. Also, there was a marked decrease in the genetic purity in subsequent classes of seed viz., foundation, certified and truthfully labeled seeds which can be assessed rapidly and efficiently by molecular marker-based assays. The results suggested that molecular markers can be used complementary to GOT assays.
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