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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3307-3318
DOI: https://doi.org/10.20546/ijcmas.2017.608.395


Development of Molecular IDS for the Elite Indian Rice Varieties and Marker-Based Assessment of Seed Purity
Mrinali Mandape1*, K. Keshavulu1, R.M. Sundaram2 and Pranathi Reddy2
1Division of Seed Science and Technology, Professor Jayashankar Telangana State Agriculture University (formerly, ANGRAU), Hyderabad 500030, Telangana, India
2Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar,Hyderabad 500030, Telangana, India
*Corresponding author
Abstract:

The study intended to characterize fifteen elite Indian rice varieties with the help of molecular markers, to develop molecular ID and to analyze the genetic purity of four selected varieties viz., MTU 1010, MTU 7029, BPT 5204 and Improved Samba Mashuri in different seed lots through Grow-Out Test (GOT) and molecular marker-based assays. The results of both assays were also compared. A total of 48 SSR markers distributed across the twelve rice chromosomes were employed producing 69 alleles which amplified among the fifteen rice genotypes. The Polymorphic Information Content (PIC) value ranged from 0.03 to 0.64. The primers RM24888 with an allele size of 200 bp uniquely identified the variety MTU 1010 from others. The marker combination of RM206 and ESSR12-20.2 with an allele size of 450 bp and 170 bp respectively, identified the variety MTU 7029 uniquely. The marker combination of RM10001 and RM206 with allele size of 480 bp and 450 bp respectively, identified the variety BPT 5204 uniquely. The marker combination of JGT06-6.81 and RM22266 with allele size of 450 bp and 200 bp respectively, identified the variety Improved Samba Mashuri uniquely. Thus, these marker or marker combination served as molecular IDs. On assessing foundation and certified seed lots (truthfully labeled in case of Improved Samba Mashuri) for genetic purity through GOT and molecular markers, impurity percentage detected through molecular markers was 0.75 to 2.75%. Also, there was a marked decrease in the genetic purity in subsequent classes of seed viz., foundation, certified and truthfully labeled seeds which can be assessed rapidly and efficiently by molecular marker-based assays. The results suggested that molecular markers can be used complementary to GOT assays.


Keywords: Genetic purity, Elite Indian rice, GOT, EST-SSRs.
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How to cite this article:

Mrinali Mandape, K. Keshavulu, R.M. Sundaram and Pranathi Reddy. 2017. Development of Molecular IDS for the Elite Indian Rice Varieties and Marker-Based Assessment of Seed PurityInt.J.Curr.Microbiol.App.Sci. 6(8): 3307-3318. doi: https://doi.org/10.20546/ijcmas.2017.608.395