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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 713-719
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.082


Antigenotoxic Effect of Withania somnifera (Ashwagandha) Extract Against DNA Damage Induced by Hydrogen Peroxide in Cultured Human Peripheral Blood Lymphocytes
Neeraj Kumar1, Anita Yadav1*, Ranjan Gupta2 and Neeraj Aggarwal3
1Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India
2Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India
3Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana, India
*Corresponding author
Abstract:

Hydrogen peroxide is well known to produce reactive oxygen species which cause damage to nucleic acids, proteins and disintegrate plasma membrane. It is also responsible for aging and neoplasmic progressions. The present study investigated the antigenotoxic effect of Withania somnifera extract against DNA damage induced by hydrogen peroxide in cultured human peripheral blood lymphocytes (PBL) using sister chromatid exchange (SCE) assay as a biomarker of genotoxicity. Heparinised fresh blood from healthy individuals was treated with hydrogen peroxide in presence of Withania somnifera extract in order to observe its antigenotoxic potential. A significant (p<0.05) dose dependent increase in frequency of SCEs was observed as the concentration of hydrogen peroxide increases. However, Withania somnifera (35 µg/ml and 70 µg/ml) in presence of 20 µM of hydrogen peroxide had significantly reduced (p<0.05) the frequencies of SCEs as compared to PBL exposed to hydrogen peroxide only. Hence, our findings suggest that Withania somnifera can protect against DNA damage induced by hydrogen peroxide in cultured human PBL.


Keywords: Hydrogen peroxide,Withania somnifera,Sister Chromatid Exchanges,DNA damage, Peripheral blood lymphocytes.
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How to cite this article:

Neeraj Kumar, Anita Yadav, Ranjan Gupta and Neeraj Aggarwal. 2016. Antigenotoxic Effect of Withania somnifera (Ashwagandha) Extract Against DNA Damage Induced by Hydrogen Peroxide in Cultured Human Peripheral Blood LymphocytesInt.J.Curr.Microbiol.App.Sci. 5(4): 713-719. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.082