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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3233-3245
DOI: https://doi.org/10.20546/ijcmas.2017.608.386


Fresh Farm Vegetables as a Source of Virulent Drug Resistant Salmonella enterica
B. Sood1*, P. Sahota1 and M. Hunjan2
1Department of Microbiology
2Department of Plant Pathology, Punjab Agricultural University, Ludhiana-141004, India
*Corresponding author
Abstract:

Plants are well known as vectors for transmission of human enteric pathogens and exaggerate the outbreak events. Salmonella enterica, rod shaped, facultative anaerobic bacteria interferes with the microbial safety of raw vegetables. This study explored three sources: Village fields, Supermarkets and Street Vendors to ensure the microbial risk among seven raw vegetables (N=725). Water samples of the hand pumps sourced from village fields showed total coliforms >10ml-1 (75.6-98.5%) and faecal coliforms >10ml-1 (59.6-91.2%). The highest Aerobic Mesophilic count (6.66 log cfu g-1) in spinach samples and highest Salmonella enterica count (5.09 log cfu g-1) in tomato samples procured from vendors was observed. Resistance to Lyncosamides, Tetracycline, Imipenem, Metronidazole and Cloxacillin has been observed. Twenty isolates were found in high Multiple Antibiotic Resistance (MAR) index range 0.36-0.8. Polymerase Chain Reaction (PCR) analysis has revealed the presence of sipB (100%), sopB (100%), spiA (100%), invA (100%) except hilA gene which was found absent in all isolates. The in-vivo expression of these virulence genes and resistance to multiple antimicrobials can potentially threat the healthy being of consumers.


Keywords: MAR, Salmonella enterica, Coliforms, Village field, Virulence genes.
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How to cite this article:

Sood, B., P. Sahota and Hunjan, M. 2017. Fresh Farm Vegetables as a Source of Virulent Drug Resistant Salmonella Enterica.Int.J.Curr.Microbiol.App.Sci. 6(8): 3233-3245. doi: https://doi.org/10.20546/ijcmas.2017.608.386