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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 3081-3087
DOI: https://doi.org/10.20546/ijcmas.2017.608.369


Effect of Soil Temperature, Moisture and pH on Soil L-glutaminase Activity
G. Pavani*, P. Chandrasekhar Rao, G. Padmaja and B. Naveen Kumar
Department of Soil Science and Agricultural Chemistry, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad – 500030, Telangana, India
*Corresponding author
Abstract:

The experiment was carried out in the department of soil science and agricultural chemistry, College of Agriculture, Rajendranagar, PJTSAU. The enzyme L-Glutaminase (L-glutamine amido hydrolase E.C. 3.5.1.2) cause the hydrolysis of L-glutamine to ammonia, L-glutamic acid, thus it makes the amide form of nitrogen available to the plants. We have detected this enzyme in soils. A simple, precise, rapid and sensitive method was used to assay its activity which involves determination of the NH+4 released by l-glutaminase activity when soil is exposed to l-glutamine, Tris (hydroxymethyl) aminomethane (THAM) buffer and toluene at 37°C for 2 h. The NH+4-N released is determined by treatment of the soil sample with 2.5 m KCl containing a l-glutaminase inhibitor (Ag2SO4) and steam distillation of an aliquot of the resulting soil suspension (Frankenberger and Tabatabai, 1991). The aim of this research work was to determine the effect of moisture, pH, and temperature on L-glutaminase activity. The results showed that the optimum temperature, moisture, pH, for L-glutaminase activity was at 50oC, 50%, pH of 8 respectively. The optimum conditions were suitable for enzyme activity, any deviation from these optimum conditions were led to lower in enzyme activity.


Keywords: L-glutaminase, Temperature, pH, Moisture, Enzyme activity.
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How to cite this article:

Pavani, G., P. Chandrasekhar Rao, G. Padmaja and Naveen Kumar, B. 2017. Effect of Soil Temperature, Moisture and pH on Soil L-glutaminase ActivityInt.J.Curr.Microbiol.App.Sci. 6(8): 3081-3087. doi: https://doi.org/10.20546/ijcmas.2017.608.369