National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial blight is one of the most important disease of Pomegranate caused by bacterium Xanthomonas axonopodis pv. punicae (Xap). The disease is characterized by small, irregular, translucent, water soaked spots with light to dark necrotic centre surrounded by prominent yellow margin on leaves. The isolates were made from the symptomatic samples collected from four districts of Karnataka viz., Vijayapur, Bagalkot, Koppal, and Chitradurg. The variability was studied among the four isolates of Xanthomonas axonopodis pv. punicae by using morphological, cultural and molecular characteristics. Xanthomonas axonopodis pv. punicae pure culture was isolated and confirmed its pathogenicity on susceptible variety Bhagawa and molecular identification was carried out using specific marker genes viz., 16S rRNA and gyrB. All four isolates were screened with three different markers viz., REP, ERIC and BOX to distinguish among them at genetic level. Amplification of ERIC, BOX and Rep region DNA from four isolates of pomegranate bacterial blight from four locations with three different primers yielded a total 28, 20 and 24 bands respectively with monomorphic banding pattern in all four isolates and amplicon size ranging from 300 to 2000 bp with BOX and ERIC primers, whereas in case of Rep primers the range is 200 to 1000 bp only. From this, it is confirmed that not much variation is existing in the collected isolates. Hence, isolate from Vijayapur (XapI) was used for further screening of the genotypes against bacterial blight both in vivo and in vitro.
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