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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2763-2775
DOI: https://doi.org/10.20546/ijcmas.2017.608.330


Characterization and Safety Assessment of Herbal Formulation (CSW/AKS/2010) in Wistar Rats: A Possible Ameliorating Remedy against Mycotoxicosis
Gauri A. Chandratre1*, A.G. Telang2, Prarabdh C. Badgujar3, Sachin S. Raut4 and A.K. Sharma5
1Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, Haryana 125004, India
2Toxicology laboratory, Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India
3Food Toxicology Section, Department of Food Science and Technology, National Institute of Food Technology Entrepreneurship and Management, Kundli, Sonipat, Haryana 131 028, India
4Animal facility, CSIR- Institute of Microcrobial Technology (IMTECH),Chandigarh-160036, India
5Mycotic and Mycotoxic Diseases Laboratory, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India
*Corresponding author
Abstract:

CSW/AKS/2010 is a novel herbal formulation consisting of Withania somnifera and Curcuma longa along with toxin binder yeast- Saccharomyces cereviciae, intended to be incorporated in livestock feed for prevention and treatment of mycotoxicoses. The present study evaluated potential safety of CSW/AKS/2010 following acute and subacute exposure in Wistar rats. Phytochemical analysis of Withania somnifera and Curcuma longa was carried out with the help of HPLC and HPTLC respectively. In acute toxicity study, single dose of 1500, 3000 and 5000 mg/kg body weight was administered by oral gavage in Wistar rats to determine its oral LD50. In subacute toxicity study, herbal formulation was fed at the dose of 5 g/kg, 10 g/kg and 15 g/kg of feed for 28 days to male Wistar rats. Hemato- biochemical parameters and pathomorphological changes in vital organs were evaluated at the end of the experiment. The HPLC fingerprint of the extract showed a spectrum profile characteristic of withanolides and HPTLC chromatogram showed high concentration of curcumin. The oral LD50 of the present formulation was estimated to be 5000 mg/kg b.w. Body weight gain was normal in treated rats and no adverse effects were obseverd. Following subacte (28 day repeated exposure), there were no significant alterations in the above mentioned parameters between treatment and control groups. The treated animals exhibited normal feed intake and dose dependent enhancing effect on growth. CSW/AKS/2010 herbal formulation could be considered safe. No observed adverse effect level of the herbal formulation was 15 g/kg of feed.


Keywords: Herbal formulation, Livestock feed, Phyto-chemical characterization, acute toxicity, sub-acute toxicity
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How to cite this article:

Gauri A. Chandratre, A.G. Telang, Prarabdh C. Badgujar, Sachin S. Raut, and Sharma, A.K. 2017. Characterization and Safety Assessment of Herbal Formulation (CSW/AKS/2010) in Wistar Rats: A Possible Ameliorating Remedy against Mycotoxicosis.Int.J.Curr.Microbiol.App.Sci. 6(8): 2763-2775. doi: https://doi.org/10.20546/ijcmas.2017.608.330