National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
CSW/AKS/2010 is a novel herbal formulation consisting of Withania somnifera and Curcuma longa along with toxin binder yeast- Saccharomyces cereviciae, intended to be incorporated in livestock feed for prevention and treatment of mycotoxicoses. The present study evaluated potential safety of CSW/AKS/2010 following acute and subacute exposure in Wistar rats. Phytochemical analysis of Withania somnifera and Curcuma longa was carried out with the help of HPLC and HPTLC respectively. In acute toxicity study, single dose of 1500, 3000 and 5000 mg/kg body weight was administered by oral gavage in Wistar rats to determine its oral LD50. In subacute toxicity study, herbal formulation was fed at the dose of 5 g/kg, 10 g/kg and 15 g/kg of feed for 28 days to male Wistar rats. Hemato- biochemical parameters and pathomorphological changes in vital organs were evaluated at the end of the experiment. The HPLC fingerprint of the extract showed a spectrum profile characteristic of withanolides and HPTLC chromatogram showed high concentration of curcumin. The oral LD50 of the present formulation was estimated to be 5000 mg/kg b.w. Body weight gain was normal in treated rats and no adverse effects were obseverd. Following subacte (28 day repeated exposure), there were no significant alterations in the above mentioned parameters between treatment and control groups. The treated animals exhibited normal feed intake and dose dependent enhancing effect on growth. CSW/AKS/2010 herbal formulation could be considered safe. No observed adverse effect level of the herbal formulation was 15 g/kg of feed.
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