National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study was carried out to establish efficient and reliable in vitro propagation protocol for Gisela 5 (Prunus cerasus x Prunus canescens) - clonal cherry rootstock. Axillary and terminal buds of ‘Gisela 5’ were collected in the spring and cultured on MS medium. After the in vitro cultures were sufficiently established, experiments were conducted to assess the influence of different levels of benzyladenine (BA), kinetin (Kin), thidiazuron (TDZ), gibrellic acid (GA3) and indole-3-butyric acid (IBA) on in vitro shoot multiplication. Data was collected on multiplication rate, shoot number, shoot length, per cent rooting, root number and root length after an interval of six weeks. Shoot multiplication and shoot length showed an increase with the increase in number of passages of subculturing which increased to a maximal of 1:10 and 6.5 cm after fourth passage. Maximum in vitro rooting (100%) was achieved on full strength MS medium fortified with 0.5 mg/l IBA whereas, very less rooting was achieved on full strength solid MS medium fortified with IAA and NAA. The Highest number of roots and root length observed on full strength solid MS medium supplemented with 0.5 mg/l IBA followed by 1.0 mg/l NAA and 0.5 mg/l IAA. Rooted plantlets were transplanted in sterilized sand for hardening and kept in the glasshouse, where 90 per cent survival was observed after 4 weeks of transfer.
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