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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 2617-2624
DOI: https://doi.org/10.20546/ijcmas.2017.608.311


An Efficient Method for In Vitro Propagation of Gisela 5 (Prunus cerasus X Prunus canescens) - Clonal Cherry Rootstock
Vishal Sharma*, Manisha Thakur and Akhil Kumar
Department of Biotechnology, Dr Yashwant Singh Parmar University of Horticulture and Forestry Nauni, Solan (HP), 173230, India
*Corresponding author
Abstract:

A study was carried out to establish efficient and reliable in vitro propagation protocol for Gisela 5 (Prunus cerasus x Prunus canescens) - clonal cherry rootstock. Axillary and terminal buds of ‘Gisela 5’ were collected in the spring and cultured on MS medium. After the in vitro cultures were sufficiently established, experiments were conducted to assess the influence of different levels of benzyladenine (BA), kinetin (Kin), thidiazuron (TDZ), gibrellic acid (GA3) and indole-3-butyric acid (IBA) on in vitro shoot multiplication. Data was collected on multiplication rate, shoot number, shoot length, per cent rooting, root number and root length after an interval of six weeks. Shoot multiplication and shoot length showed an increase with the increase in number of passages of subculturing which increased to a maximal of 1:10 and 6.5 cm after fourth passage. Maximum in vitro rooting (100%) was achieved on full strength MS medium fortified with 0.5 mg/l IBA whereas, very less rooting was achieved on full strength solid MS medium fortified with IAA and NAA. The Highest number of roots and root length observed on full strength solid MS medium supplemented with 0.5 mg/l IBA followed by 1.0 mg/l NAA and 0.5 mg/l IAA. Rooted plantlets were transplanted in sterilized sand for hardening and kept in the glasshouse, where 90 per cent survival was observed after 4 weeks of transfer.


Keywords: In vitro, Clonal cherry rootstock, Glasshouse.
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How to cite this article:

Vishal Sharma, Manisha Thakur and Akhil Kumar. 2017. An Efficient Method for In Vitro Propagation of Gisela 5 (Prunus Cerasus X Prunus Canescens) - Clonal Cherry Rootstock.Int.J.Curr.Microbiol.App.Sci. 6(8): 2617-2624. doi: https://doi.org/10.20546/ijcmas.2017.608.311