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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 1523-1533
DOI: https://doi.org/10.20546/ijcmas.2017.608.183


Study of the Optimal Physical Parameters and Action of the Proteolytic Enzyme on the Antimicrobial and Antioxidant Potential of the Protein Isolated from Different Pulses
Shahana Jabi1* and Abhishek Mathur2
1Department of Biotechnology, Himalayan University, Arunachal Pradesh, India
2R&D cum New product Development, EBEC-NCFT, New Delhi, India
*Corresponding author
Abstract:

In the present study, proteins were extracted and purified from germinated seeds viz. Vigna radiata (mung bean), Cicer arietinum (gram white and gram black). The protein extracted was evaluated for any antimicrobial effects against the different pathogens and to determine antioxidant potential. The studies were performed to assess the effect of pH, temperature and proteolytic enzymes on the antimicrobial nature of the protein extracted. It was found that the extracted antimicrobial protein had prominent antimicrobial effect on the different microbial pathogens at the range of pH 4-7. The studies also showed the strong antioxidant potential of the different seeds protein isolated and purified. It was also observed that the temperature from 25ºC-30ºC has no adverse effect on the antimicrobial action of the proteins extracted. The results of the present study thus help to explore and formulate the potent antimicrobial agent against different variety of pathogens and to formulate strong antioxidant agent.


Keywords: Antimicrobial potential, Antioxidant potential, Cicer arietinum, protein, Vigna radiata.
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How to cite this article:

Shahana Jabi and Abhishek Mathur. 2017. Study of the Optimal Physical Parameters and Action of the Proteolytic Enzyme on the Antimicrobial and Antioxidant Potential of the Protein Isolated from Different Pulses.Int.J.Curr.Microbiol.App.Sci. 6(8): 1523-1533. doi: https://doi.org/10.20546/ijcmas.2017.608.183