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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 1380-1387
DOI: https://doi.org/10.20546/ijcmas.2017.608.168


Comparative Structure Modeling of Glyoxalase II Protein of Oryza sativa
Ramandeep Kaur Jhinjer*, Naveen Duhan and Parveen Chhuneja
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab
*Corresponding author
Abstract:

Glyoxalase I (gly I) and Glyoxalase II (gly II), zinc-binding enzymes of the glyoxalase pathway, carry out catabolism of methylglyoxal (MG). Gly II gene cloned from Oryza sativa has been widely used in genetic transformation experiments for salinity tolerance. In the present study, we tried to propose a potential model of the Oryza sativa gly II protein generated via SWISS-MODEL, CPH models 3.0 Server, I-TASSER, HHPred and MODELLER software. Thirteen different models were constructed using different tools and were further optimized by performing Energy minimization and Loop building techniques. The final model was suggested after analyzing and validating the structure by using Structural Analysis and Verification Server (SAVS). Further functional characterization of the modeled protein was accomplished by tool COFACTOR which involves prediction of functional active site region and binding site region. This model can be used for further analysis for different studies.


Keywords: Glyoxalase II protein, Oryza sativa, Homology Modeling, Swiss-PdbViewer, SAVS
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How to cite this article:

Ramandeep Kaur Jhinjer, Naveen Duhan and Parveen Chhuneja. 2017. Comparative Structure Modeling of Glyoxalase II Protein of Oryza sativa.Int.J.Curr.Microbiol.App.Sci. 6(8): 1380-1387. doi: https://doi.org/10.20546/ijcmas.2017.608.168