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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 608-614
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.069


Studies on Microbial Diversity of a Soda Lake in India by inogradsky Column Technique
Harshil H. Bhatt1, Bhimaraj M. Sharma2 and Vivek N. Upasani3*
Department of Biotechnology, Kadi SarvaVishwavidyalaya, Gandhinagar 382015, India
Fisheries Officer, Commissioner of Fisheries, Bharuch, India.
Department of Microbiology, M. G. Science Institute, Navrangpura, Ahmedabad 380009, India
*Corresponding author
Abstract:

Halophilic organisms are found in habitats with a high concentration of salt (mainly NaCl) such as the Great Salt Lake, Utah; Owens Lake, California; the Dead Sea, soda lakes such as Lake Magadii, Kenya and Sambhar Salt Lake, India; marine salterns across the globe, etc. Organisms living in such habitat require high salt concentration (5-25% NaCl) and/or alkaline pH for growth. Algae, archaea, purple and green sulfur bacteria are the dominant microflora of these extreme habitat. Samples were collected from Sambhar Lake and microbial diversity was examined by cultivating halophilic organisms in Winogradsky Column. Successive changes in the column lead to changes in diversity also. Here we report on microbial diversity based on photomicrography and t-RFLP method. This is the first report on the use of Winogradsky column technique for enrichment and study the microbial diversity of Sambhar Salt Lake.


Keywords: Microbial diversity,soda lake, Sambhar Salt Lake (SSL), Winogradsky Column, t-RFLP.
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How to cite this article:

Harshil H. Bhatt, Bhimaraj M. Sharma and Vivek N. Upasani. 2016. Studies on Microbial Diversity of a Soda Lake in India by Winogradsky Column Technique.Int.J.Curr.Microbiol.App.Sci. 5(4): 608-614. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.069