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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:8, August, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 1361-1369
DOI: https://doi.org/10.20546/ijcmas.2017.608.166


Antimicrobial Peptide Genes Present in Indigenous Isolates of Bacillus spp. Exhibiting Antimicrobical Properties
Tamalika Sarangi1*, S. Ramakrishnan1 and S. Nakkeeran2
1Department of Nematology
2Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore – 641003, India
*Corresponding author
Abstract:

An attempt was made to detect the presence of antimicrobial peptide genes (AMPs) in ten different indigenous isolates of Bacillus spp. proved to be effective against M. incognita, Fusarium oxysporum f.sp.lycopersici and their disease complex. The study revealed the presence of iturin A, iturin C, iturin D, surfactin, bacilomycin D, fengycin D, zwitermycin, bacillomycin bac D, bacilysin bcc AB and fengycin CAE in the above isolates of Bacillus spp. Among the ten different AMPs, the iturin A gene was found to present in all the ten isolates of Bacillus spp. Of all indigenous isolates B. thuringiensis (TLBRE2), B. cereus (CLB2D) were in possession of higher number of eight AMPs. It was followed by B. tequilensis (TLB2) with seven genes, B. licheniformis and B. Cereus (TSB 3, CLB2 and TSB4D) with six genes, B. weihenstephanensis (CLB3) and B. subtilis (TLBRE1) with four genes and B. subtilis (TSB5) with three genes.


Keywords: Bacillus spp, Polymerase Chain Reaction, Antimicrobial peptide genes, Zwittermycin, Bacilysin bacD, Bacilysin bacAB, Fengycin CAE.

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How to cite this article:

Tamalika Sarangi, S. Ramakrishnan and S. Nakkeeran. 2017. Antimicrobial Peptide Genes Present in Indigenous Isolates of Bacillus Spp. Exhibiting Antimicrobical Properties.Int.J.Curr.Microbiol.App.Sci. 6(8): 1361-1369. doi: https://doi.org/10.20546/ijcmas.2017.608.166
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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