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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 1325-1329
DOI: https://doi.org/10.20546/ijcmas.2017.608.161


Tube Adherence Test as a Screening Tool for Detection of Biofilm Formation among Staphylococcus aureus
K.Ram Mohan Reddy*
Rajiv Gandhi Institute of Public Health and Center for Disease Control, 426/27, 33rd Cross, 18th Main, 4th T Block, Jayanagar, Bangalore -560 041, India
*Corresponding author
Abstract:

In view of large number of infections caused by biofilm forming organism, a reliable method for its detection is necessary. Highly accurate methods like Polymerase Chain Reaction (PCR) are available for detection of biofilm, but these are beyond the scope of majority of microbiology laboratories in developing nations like India. To test reliability of Tube Adherence test as a screening tool for detection of biofilm formation among Staphylococcus aureus. With universal safety precautions various clinical samples were collected from patients with indwelling medical device for more than 48 hours. Screening for biofilm detection was done using Tube adherence method and tissue culture plate method. Tube method was 97.5% sensitive and 100% specific for detection of biofilm formation. Tube method can be used as a general screening method for detection of biofilm producing bacteria in laboratories.


Keywords: Biofilm, Staphylococcus aureus, Tube method, Specificity.
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How to cite this article:

Ram Mohan Reddy, K. 2017. Tube Adherence Test as a Screening Tool for Detection of Biofilm Formation among Staphylococcus aureus.Int.J.Curr.Microbiol.App.Sci. 6(8): 1325-1329. doi: https://doi.org/10.20546/ijcmas.2017.608.161