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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 1140-1147
DOI: https://doi.org/10.20546/ijcmas.2017.608.141


Determination of Minimum Inhibitory Concentration of Liposomes: A Novel Method
Atulya Manuel1* and Nabeel Abdulrahman2  
1CCS National Institute of Animal Health, Baghpat, Uttar Pradesh, India
2College of Pharmacy, Qatar University, Doha, Qatar
*Corresponding author
Abstract:

For determination of minimum inhibitory concentration (MIC) of antimicrobial agents the Clinical and Laboratory Standards Institute (CLSI) guidelines are considered as the golden standards. Unfortunately, there is no standard guideline for MIC determination of liposomal and nano-formulations loaded with antimicrobial agents in fluid medium. In this study, a new method has been developed and standardized for determining MIC of suspensions, which is the major limitation of MIC determination in fluid media. The new method makes use of the fluorescent dye resazurin for testing MIC. The fluorescence assay was standardized and was found equally valid as that of the conventional method. The CLSI broth microdilution method and the microtiter plate based resazurin assay were evaluated for the liposomal MIC determination. The fluorescence assay was found to be highly feasible, repeatable and cost effective. The assay was found to have high sensitivity, specificity, positive and negative predictive value. Even though the sensitivity and negative predictive value of broth microdilution assay were found good, specificity and positive predictive value were low especially in case of the tested Pseudomonas aeruginosa strain. Hence the new fluorescence assay is a valid test for liposomal MIC determination and can replace the broth microdilution assay.


Keywords: Broth microdilution assay, Liposome, Resazurin, Fluorescence assay, Suspensions, Antimicrobial assay.
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How to cite this article:

Atulya Manuel and Nabeel Abdulrahman. 2017. Determination of Minimum Inhibitory Concentration of Liposomes: A Novel MethodInt.J.Curr.Microbiol.App.Sci. 6(8): 1140-1147. doi: https://doi.org/10.20546/ijcmas.2017.608.141