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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 565-577
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.064


Phenotypic detection of Extended Spectrum Beta- Lactamase producing uropathogens using DDST, PCT, Chrom agar and E-test – A comparitive study
R. Prabha*, Joshy M. Easow and M. Swapna
Veeramamunivar street, Radhakrishnan nagar, Pondicherry 605009, India
*Corresponding author
Abstract:

Urinary tract infections (UTI) are a serious threat to human health affecting millions of people every year resulting in high morbidity and mortality. Enterobacteriaceae species like Escherichia coli, Proteus mirabilis, Enterobacter aerogenes, Citrobacter freundii and Klebsiella pneumoniae are the commonest uropathogens implicated in bacteremia and nosocomial infections. Aims and objectives: To identify and study the incidence of extended spectrum beta-lactamase producing Enterobacteriaceae in urinary tract infections by different phenotypic detection tests. About 100 Uropathogens isolated from urine samples of inpatients and outpatients of SVMCH during 2014-2015 were included. ESBL producing isolates were identified by screening methods and confirmed by phenotypic methods like DDST, PCT, E-test and CHROM agar. Among 52 isolates of E. coli 40% was ESBL producer, among 4 isolates of Citrobacter 50% was ESBL producer, out of 5 isolates of P. vulgaris 20% was ESBL producer, out of 6 isolates of K. oxytoca 33% was ESBL producer, out of 13 isolates of P. mirabilis 23% was ESBL producer, out of 16 isolates of K. pneumoniae 25% was ESBL producer, out of 3 isolates of E. aerogenes 67% was ESBL producer and none of the isolates of M. morgagnii was ESBL producer. By DDST, 29% of ESBL producing isolates and 71% of non-ESBL isolates were detected. With PCT, E-test and ESBL CHROM agar 35% of ESBL producers and 65% of non-ESBL isolates were detected. Conclusion: For early detection of ESBL producers, methods like DDST, PCT can be performed in microbiology laboratories as routine test, as they are simple to perform and cost effective. Those isolates which are found to be ESBL producers by screening methods can be confirmed by tests like E-test, ESBL CHROM agar which are found to be equally effective in detecting ESBL producers.


Keywords: ESBL; Extended Spectrum Beta Lactamase, DDST – Double Disc Synergy Test, PCT; Phenotypic Confirmatory Test, E-test – Epsilomer test.
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How to cite this article:

Prabha, R., Joshy M. Easow and Swapna, M. 2016. Phenotypic detection of Extended Spectrum Beta- Lactamase producing uropathogens using DDST, PCT, Chrom agar and E-test – A comparitive studyInt.J.Curr.Microbiol.App.Sci. 5(4): 565-577. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.064