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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 553-564
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.063


Prevalence, Antimicrobial Susceptibilities and Molecular Characterization of Enteric Bacterial Pathogens Isolated from Patients with Infectious Diarrhoea in Cairo, Egypt
Abdel-Nasser A. El-Moghazy, Mahmoud M. Tawfick* and Mahmoud M. El-Habibi
Microbiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
*Corresponding author
Abstract:

Infectious diarrhoea continues to cause significant morbidity and mortality, particularly among children worldwide. This problem is especially acute in developing countries. This study aimed to determine the prevalence, antimicrobial susceptibilities and plasmid-profile based molecular characterization of enteric bacteria isolated from patients with infectious diarrhoea in Cairo, Egypt. A total of 465 bacterial isolates were isolated from 412 diarrhoea faecal specimens and swabs collected from children and adults patients presenting acute diarrhoea, during the period from December 2013 to December 2015, at three hospitals in Cairo, Egypt. The fecal specimens were cultured by conventional methods for enteric bacterial pathogens. The biochemically identified bacterial isolates were subjected to the antimicrobial susceptibility testing using Kirby-Bauer disc diffusion method. For multi-drug resistant (MDR) isolates, identification and antimicrobial susceptibility pattern was confirmed by the automated MicroScan Walkaway system. Plasmid DNA was isolated from MDR isolates and different profiles were assessed by agarose gel electrophoresis. It was found that E. coli represents the most prevalent bacterial species among isolates (46.7 %), followed by K. pnemoniae (29.9 %), P. vulgaris (14.3 %), Enterobacter spp. (5.3 %), P. mirabilis (4.4 %), Citrobacter spp. (1.7 %) and Shigella spp. (0.7 %). Isolates in this study showed high levels of multidrug antimicrobial resistance to the antimicrobials tested as 46.9 % of isolates were MDR. The plasmid profile analysis revealed that plasmids were diverse and distributive among isolates, thus different patterns were detected. In conclusion, this study revealed that infectious diarrhoea is attributed to different bacterial species, with E. coli is the most prevalent one. Most of isolated enteric bacterial pathogens are MDR, which could be explained by the indiscriminate and widespread use of antimicrobial agents and the empirical antibiotic therapy in hospitals. The diverse patterns of plasmid profiles observed could be due to higher exposure of people to enteric bacterial pathogens in these places. Thus, regular surveillance for the etiological agents of infectious diarrhoea to monitor the changing epidemiology of these resistant bacteria is warranted.


Keywords: Enteric bacteria, Antimicrobial resistance, Infectious diarrhoea,Plasmid profile.
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How to cite this article:

Abdel-Nasser A. El-Moghazy, Mahmoud M. Tawfick and Mahmoud M. El-Habibi. 2016. Prevalence, Antimicrobial Susceptibilities and Molecular Characterization of Enteric Bacterial Pathogens Isolated from Patients with Infectious Diarrhoea in Cairo, EgyptInt.J.Curr.Microbiol.App.Sci. 5(4): 553-564. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.063