National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out to envisage the impact of chlorpyrifos on gut bacterial population of E. fetida. The LC50 of chlorpyrifos was analyzed by 24 hour paper contact toxicity test as recommended by OECD. The LC50 of chlorpyrifos for 24 hours is 0.25%. Then the worms were exposed to the LC50 dose, one dose lower (0.125%) and one dose higher (0.375%) of chlorpyrifos. The samples of gut from treated worms were cultured on nine different culture media viz. Mac Conkey Agar, LB media, R.S. Media, King’s B media, YEMA, Jensen media, Malatemedia, Endo agar media and nutrient agar media. Significant differences in total viable bacterial count on Luria Bertani and King’s media has been observed in earthworms exposed to lowest dose (T1) of chlorpyrifos as compared to control. However, when the worms were exposed to highest dose (T3) significant reduction in total viable counts was observed in comparison to control except on Endo Agar media. It may also be noted that greater number of viable counts was observed in vermicompost (VC) samples as compared to those present in worm’s gut.
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