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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 824-830
DOI: https://doi.org/10.20546/ijcmas.2017.608.104


Enumeration of Gut Bacterial Population in Eisenia fetida Exposed to Chlorpyrifos
Jyoti Yadav1*, Dharambir Singh1, Swati Sindhu2, Rajesh Gera2 and Shefali1
1Department of Zoology,
2Department of Microbiology,CCS Haryana Agricultural University, Hisar- 125004, India
*Corresponding author
Abstract:

The present study was carried out to envisage the impact of chlorpyrifos on gut bacterial population of E. fetida. The LC50 of chlorpyrifos was analyzed by 24 hour paper contact toxicity test as recommended by OECD. The LC50 of chlorpyrifos for 24 hours is 0.25%. Then the worms were exposed to the LC50 dose, one dose lower (0.125%) and one dose higher (0.375%) of chlorpyrifos. The samples of gut from treated worms were cultured on nine different culture media viz. Mac Conkey Agar, LB media, R.S. Media, King’s B media, YEMA, Jensen media, Malatemedia, Endo agar media and nutrient agar media. Significant differences in total viable bacterial count on Luria Bertani and King’s media has been observed in earthworms exposed to lowest dose (T1) of chlorpyrifos as compared to control. However, when the worms were exposed to highest dose (T3) significant reduction in total viable counts was observed in comparison to control except on Endo Agar media. It may also be noted that greater number of viable counts was observed in vermicompost (VC) samples as compared to those present in worm’s gut.


Keywords: Bacteria, Chlorpyrifos, Insecticide, Eisenia fetida, CFU.
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How to cite this article:

Jyoti Yadav, Dharambir Singh, Swati Sindhu, Rajesh Gera and Shefali. 2017. Enumeration of Gut Bacterial Population in Eisenia fetida Exposed to Chlorpyrifos.Int.J.Curr.Microbiol.App.Sci. 6(8): 824-830. doi: https://doi.org/10.20546/ijcmas.2017.608.104