National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To investigate the potential ability of two genera of the Streptosporangiaceae family to act as biocatalysts, we screened 13 Streptosporangium and 13 Nonomuraea strains. Five recommended media (227, 228, 231, 266 and 268 media) were tested for use in the liquid culture of these actinomycetes. Three Streptosporangium strains (Streptosporangium sp. NBRC16544, Streptosporangium sp. NBRC101289, and Streptosporangium sp. NBRC101798) showed good growth when cultured in 227 and 228 media, as did three Nonomuraea strains (N. spiralis NBRC14097, N. helvata NBRC14681, and Nonomuraea sp. NBRC16543) when cultured in 227 and 266 media. Therefore, the stereoselective reduction of various carbonyl compounds using these six strains was investigated. It was found that these strains possess reducing activity towardsï¡-keto esters (aliphatic and aromatic) and an aromatic ï¡-keto amide. Based on the conversion ratios and the stereoselectivity of the produced alcohols, we suggest that Streptosporangium sp. NBRC101798 cultured in 228 medium and N. helvata NBRC14681 cultured in 266 medium are potential biocatalysts for the stereoselective reduction of ï¡-keto esters and an aromatic ï¡-keto amide to yield the corresponding chiral alcohols. In addition, our results also suggest that cryopreserved cells could be used as biocatalysts for the asymmetric reduction of carbonyl compounds.
Citations |
 |
 |
 |
 |
