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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 707-717
DOI: https://doi.org/10.20546/ijcmas.2017.608.090


Microbial Preparation of Chiral Alcohols: Stereoselective Reduction of Carbonyl Compounds using Two Genera of the Streptosporangiaceae Family - Streptosporangium and Nonomuraea
K. Ishihara1*, A. Chiba1, K. Onishi1, A. Okawa1, Y. Mizote1,D.Uesugi2, N. Masuoka2, N. Nakajima3 and H. Hamada1,2
1Department of Life Science, Okayama University of Science, Okayama, Japan
2Department of Research and Development, Meister Bio Co. Ltd., Okayama, Japan
3Department of Nutritional Science, Okayama Prefectural University, Soja, Okayama, Japan
*Corresponding author
Abstract:

To investigate the potential ability of two genera of the Streptosporangiaceae family to act as biocatalysts, we screened 13 Streptosporangium and 13 Nonomuraea strains. Five recommended media (227, 228, 231, 266 and 268 media) were tested for use in the liquid culture of these actinomycetes. Three Streptosporangium strains (Streptosporangium sp. NBRC16544, Streptosporangium sp. NBRC101289, and Streptosporangium sp. NBRC101798) showed good growth when cultured in 227 and 228 media, as did three Nonomuraea strains (N. spiralis NBRC14097, N. helvata NBRC14681, and Nonomuraea sp. NBRC16543) when cultured in 227 and 266 media. Therefore, the stereoselective reduction of various carbonyl compounds using these six strains was investigated. It was found that these strains possess reducing activity towards-keto esters (aliphatic and aromatic) and an aromatic -keto amide. Based on the conversion ratios and the stereoselectivity of the produced alcohols, we suggest that Streptosporangium sp. NBRC101798 cultured in 228 medium and N. helvata NBRC14681 cultured in 266 medium are potential biocatalysts for the stereoselective reduction of -keto esters and an aromatic -keto amide to yield the corresponding chiral alcohols. In addition, our results also suggest that cryopreserved cells could be used as biocatalysts for the asymmetric reduction of carbonyl compounds.


Keywords: Biocatalyst, Stereoselective reduction, Chiral alcohol, Streptosporangium, Nonomuraea, Cryopreserved cells
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How to cite this article:

Ishihara, K., A. Chiba, K. Onishi, A. Okawa, Y. Mizote, D. Uesugi, N. Masuoka, N. Nakajima and Hamada, H . 2017. Microbial Preparation of Chiral Alcohols: Stereoselective Reduction of Carbonyl Compounds using Two Genera of the Streptosporangiaceae Family - Streptosporangium and Nonomuraea.Int.J.Curr.Microbiol.App.Sci. 6(8): 707-717. doi: https://doi.org/10.20546/ijcmas.2017.608.090