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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 441-447
DOI: https://doi.org/10.20546/ijcmas.2017.608.058


Isolation and Identification of Bacillus cereus from Fish and their Handlers from Jammu, India
U. Rasool, Ajaz Ahmad*, G.A. Badroo, Mir Mudasir, S. Fayaz and R. Mustafa
Department of Food Technology, Doon Valley Institute of Engineering and Technology, Karnal-132001, Haryana, India
*Corresponding author
Abstract:

The aim of the study is to know the occurrence of B. cereus and level of contamination with B. cereus in fish from Jammu region. In this study 140 samples were collected from various parts of Jammu. Out of 140 samples 44 (31.42%) samples showed characteristic peacock blue colored colonies on selective PEMBA. On Gram staining all these isolates were Gram positive rods and sometimes spores were also seen. Biochemical characterization was done to identify B. cereus isolates was done. Forty four (44) isolates were subjected to various biochemical tests but only 39 (27.85%) isolates showed characteristic biochemical features as that of B. cereus. All the 39 isolates were positive for catalase test, citrate utilization test and motility. Thirty seven (94.87%) B. cereus isolates hydrolyzed starch and produces beta-hemolysis on sheep blood agar. All the 39 Bacillus cereus isolates were positive for nitrate reduction test and Voges-Proskauer test but negative for oxidase test.


Keywords: Bacillus cereus, Enterotoxigenic, Fish, Gram staining, PEMBA.
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How to cite this article:

Rasool, U., Ajaz Ahmad, G.A. Badroo, Mir Mudasir, S. Fayaz and Mustafa, R. 2017. Isolation and Identification of Bacillus cereus from Fish and their Handlers from Jammu, India.Int.J.Curr.Microbiol.App.Sci. 6(8): 441-447. doi: https://doi.org/10.20546/ijcmas.2017.608.058