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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 395-409
DOI: https://doi.org/10.20546/ijcmas.2017.608.053


Comparative Study of Conventional Staining Techniques, Quantitative Buffy Coat, Immunochromatography Methods and Molecular Methods (Gene Amplification) for Diagnosis of Malaria
K.S. Vaisakhi1*, Sujatha Kolla2 and Achut Rao2
1Dr. V. R. K. Medical College, STAR Hospitals, Hyderabad, Telangana, India
2RVMIMS Medical College, Hyderabad, Telangana, India
*Corresponding author
Abstract:

The study showed that the immunochromatography assay tests were reliable and easy to use. Blood samples were collected from 175 patients presenting with typical features suggesting clinical malaria. These blood samples were subjected to various test which included thin and thick smears stained with JSB, Giemsa, Leishman, Fields stain and the other methods employed which included Arcidine orange staining, Quantitative Buffy Coat methods, immunochromatography strips – optimal and para HIT – f. Assay are used. Gene amplification technique (Polymerase chain reaction) was used. However, RDTS are more suited for investigator / health workers in situations where health services are different (or) absent. RDT’s in conjunction with microscopy should improve diagnosis of malaria, thus likely to contribute greatly to an effective control of malaria in resource poor countries.


Keywords: Microscopy, Quantitative buffy coat, Immuno-chromatography, PCR.
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How to cite this article:

Vaisakhi, K.S., Sujatha Kolla and Achut Rao. 2017. Comparative Study of Conventional Staining Techniques, Quantitative Buffy Coat, Immunochromatography Methods and Molecular Methods (Gene Amplification) for Diagnosis of Malaria.Int.J.Curr.Microbiol.App.Sci. 6(8): 395-409. doi: https://doi.org/10.20546/ijcmas.2017.608.053