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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 270-277
DOI: https://doi.org/10.20546/ijcmas.2017.608.036


Recovery of Resistant Thermophilic Campylobacters on Farm and Market Vegetables
A.B. Karikari1*, K. Obiri-Danso2, E.H. Frimpong3 and K.A. Krogfelt4
1Department of Clinical Microbiology, University for Development Studies Tamale, Ghana
2Department of Theoretical and Applied Biology,
3Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana,
4Department of Microbiology and Infection Control, Statens Serum Institute Denmark
*Corresponding author
Abstract:

This study focused on establishing the presence of Campylobacter at the farm and retail level and evaluated the antibiogram profile of isolated species. Campylobacter spp. were isolated on mCCDA agar and confirmed by API Campy (biomereaux, France) and the susceptibility profile determined by Kirby-Bauer disk diffusion method. A total of 124 market vegetables comprising 59 lettuce and 65 cabbages and 117 farm vegetables made up of 94 lettuce and 23 cabbages were analysed. Prevalence of 41.9% (52/124) and 23.9% (28/117) were respectively documented in market and farm vegetables. Campylobacter jejuni was the dominant species keyed out from market vegetables (61.5%) followed by C. coli (23.0%), C. lari (11.5%) and C. jejuni sub sp. doylei (3.8%). Fifty percent (50%) of farm vegetable isolates were C. jejuni, 25% constituted C. jejuni sub sp. doylei and 21.4%, 3.6% were C. coli and C. lari respectively. All isolates (100%) were multidrug resistant, with highest resistance observed against cephalexin, cefotaxime, ampicillin, erythromycin and chloramphenicol (86-100%), 64-75% against tetracyclines, 32-49% to ciprofloxacin, nalidixic acid and norfloxacin and 57-67% to trimethoprim sulphamethoxazole. Against kanamycin and gentamicin resistance of 4-46% was obtained while no resistance (0%) was observed against imipenem. The results provide baseline information on Campylobacter in vegetables and the possible risk it poses to consumers in the face of high level resistance in the species.


Keywords: Antibiotic resistance, Vegetables, Farm, market, Ghana.
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How to cite this article:

Karikari, A.B., K. Obiri-Danso, E.H. Frimpong and Krogfelt, K.A. 2017. Recovery of Resistant Thermophilic Campylobacters on Farm and Market VegetablesInt.J.Curr.Microbiol.App.Sci. 6(8): 270-277. doi: https://doi.org/10.20546/ijcmas.2017.608.036