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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 203-210
DOI: https://doi.org/10.20546/ijcmas.2017.608.028


Isolation and Identification of Klebsiella pneumoniae using API-20E analytical system and conventional PCR assay
Abeer Ghassan Munther Al-Agha1, Nazar Jabar Muslih Al-Khafaji2 and Amer Khazal Salih Al-Azawi1
1Department of Microbiology, College of Veterinary Medicine, University of Diyala,Diyala, Iraq
2Department of Medicine, College of Veterinary Medicine, University of Diyala, Diyala, Iraq
*Corresponding author
Abstract:

Klebsiella spp., particularly Klebsiella pneumoniae, are important causes of nosocomial infections due to the presence of capsular polysaccharide which is a major surface-located virulence properties associated with the pathogenesis of Klebsiella pneumoniae. The capsule is an elaborate polysaccharide matrix that encases the entire cell surface and provides resistance against many host defense mechanisms. D-galacton II has an important role in synthesising the lipopolysaccharide of Klebsiella pneumoniae and many other gram negative bacteria. This study included the isolation of Klebsiella pneumoniae bacteria from different sources in the environment and identification by using API-20E system and a conventional Polymerase chain reaction assay which detects Klebsiella pneumoniae wbbZ gene that involved in expression of D-galacton. The results of our study confirmed the positive identification of the bacteria using API-20E analytical system and also the presence of wbbZ gene in two of Klebsiella pneumoniae tissue invasive strains using conventional PCR assay.


Keywords: Klebsiella, API-20E, wbbZ gene
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How to cite this article:

Abeer Ghassan Munther Al-Agha, Nazar Jabar Muslih Al-Khafaji and Amer Khazal Salih Al-Azawi. 2017. Isolation and Identification of Klebsiella pneumoniae using API-20E analytical system and conventional PCR assay.Int.J.Curr.Microbiol.App.Sci. 6(8): 203-210. doi: https://doi.org/10.20546/ijcmas.2017.608.028