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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 459-468
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.054


Detection and Genotyping of Giardia lamblia in Clinical and Environmental Samples in Some Regions of Baghdad city
Bedir M.Abbas1, Ihsan M. AL-Saqur2 and Hadeel A. Majeed1*
 1Biology Department, College of Science, Al-Mustansiriya University, Iraq
2Technical Lab, Analysis Department, Al-Israa University College, Iraq
*Corresponding author
Abstract:

The present study was conducted to estimate the prevalence of Giardia lamblia infection in some regions of Baghdad city, the relationship of source of drinking water with the prevalence of this intestinal protozoal parasite had been evaluate, The study revealed that the total rate of G.lamblia infection in humans was 68/375 (18.13%) according to three diagnostic methods, there was significant relation (p≤0.01) between infectivity rate (25%) of this parasite and persons who consumed tap water as a source of drinking water as compared to other source of drinking water. RT-PCR tpi gene application was able to detected and genotyping G.lamblia to assemblage A and B, since mix infection of assemblage (A+B) are more prevalent in fecal samples than others, while in environmental samples the assemblage A, assemblage B showed in 1/4 (25%), 2/4(50%) respectively and mixed assemblage (A+B) showed in 1/4 (25%) of samples.


Keywords: Giardia lamblia,Genotyping,infectivity rate,RT-PCR tpi gene.
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How to cite this article:

Bedir M.Abbas, Ihsan M. AL-Saqur and Hadeel A. Majeed. 2016. Detection and Genotyping of Giardia lamblia in Clinical and Environmental Samples in Some Regions of Baghdad city.Int.J.Curr.Microbiol.App.Sci. 5(4): 459-468. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.054