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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 90-100
DOI: https://doi.org/10.20546/ijcmas.2017.608.012


Extraction and Purification of Lipopolysaccharide of Klebsiella pneumoniae Isolates
Abeer Ghassan Munther Al-Agha*
Department of Microbiology, College of Veterinary Medicine, University of Diyala, Diyala, Iraq
*Corresponding author
Abstract:

The genus Klebsiella contains a number of opportunistic pathogens which cause several serious infections, including pneumonia, bacteremia, and urinary tract infections. Characteristically, Klebsiella spp. produce large mucoid colonies because of the synthesis of large amounts of capsular polysaccharide (CPS). The LPS molecule is a virulence determinant in Klebsiella pneumoniae. In the present work from a pathogenic urine sample Klebsiella pneumoniae was obtained, the lipopolysaccharide extracted and completely purified by gel filtration chromatography. The purity was confirmed through determination of protein concentration and carbohydrate.


Keywords: Lipopolysaccharide, Klebsiella.
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How to cite this article:

Abeer Ghassan Munther Al-Agha. 2017. Extraction and Purification of Lipopolysaccharide of Klebsiella pneumoniae IsolatesInt.J.Curr.Microbiol.App.Sci. 6(8): 90-100. doi: https://doi.org/10.20546/ijcmas.2017.608.012