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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(8): 75-89
DOI: https://doi.org/10.20546/ijcmas.2017.608.011


Assessment of Metabolic Potential and Insilico Analysis of Enzymes Involved in Inducing Air Pollution Tolerance in Some Angiosperm Plants
Anamika Tripathi1, Akhil Gupta1*, Apoorv Tiwari2,3 and Anand Pathak4
1Pollution Ecology Research Laboratory, Department of Botany, Hindu College Moradabad, University of Rohilkhand, 244001, India
2Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS),Allahabad, 211007, India
3Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India
4Department of Soil Science, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145, India
*Corresponding author
Abstract:

To analyze the air pollution tolerance capacity of ten selected plant species, their biochemical analysis was done, while they were growing in ambient polluted air. For this purpose, there ambient air quality monitoring was done for one year with parameters SO2, NO2, PM10 and PM2.5, Plants species selected were Alstonia scholaris, Anthocephalus kadamba, Bauhinia variegate, Cassia fistula, Tectona grandis, Ficusrumphii, Mangifera indica, Polyalthia longifolia, Pongamia pinnata and Sarca indica. Biochemical parameters analyzed were Ascorbic acid, chlorophyll,pH, and relative water content. Results indicate that some can be used as indicators and some as a sink of air pollution. Air Pollution Tolerance Index (APTI) was calculated by calculating biochemical parameters. S. India (49.36), and Alstonia scholaris (33.66) F. rumphii (30.17) showed high; and A. kadamba (17.71), B. variegate (19.69) and Pongamia pinnata (17.72) showed intermediate response while M. indica, (12.39), P. Longifolia (11.88), Cassia fistula (9.92) and T. Grandis (9.68) showed low tolerance as calculated through achange in their biochemical parameters in response to enhanced air pollution. In silico study of enzymes involved in the air pollution tolerance in the above plants was also done and in the phylogenetic analysis of all 9 enzymes, it was found that two clusters were formed in both the cases i.e. nucleotide and protein sequences. Cluster A Superoxide dismutase and Expansion two enzymes while in Cluster B, Catalase, Peroxidases, Glycosyl-transferase, Phenylalanine ammonia lyase, Sucrose synthase, Polygalacturonase, Laccase were clustered. The insilico and biochemical analysis strongly suggested that these enzymes were highly expressed in the air pollution condition and might play a major role in the air pollution control.


Keywords: Air Pollution Tolerance Index (APTI), Biochemical parameters, SO2, NO2, PM10, PM2.5, In-silico, Domain, Phylogenetics, Moradabad.
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How to cite this article:

Anamika Tripathi, Akhil Gupta, Apoorv Tiwari and Anand Pathak. 2017. Assessment of Metabolic Potential and Insilico Analysis of Enzymes Involved in Inducing Air Pollution Tolerance in Some Angiosperm Plants.Int.J.Curr.Microbiol.App.Sci. 6(8): 75-89. doi: https://doi.org/10.20546/ijcmas.2017.608.011