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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4557-4567
DOI: https://doi.org/10.20546/ijcmas.2017.607.476


Phytochemical Screening and Antimicrobial Evaluation of Syzygium aromaticum Extract and Essential oil
Simiat Olanike Jimoh1*, Lateefah Adelanke Arowolo1 and Kazeem Adelani Alabi2
1Microbiology Unit, Department of Biological Sciences, College of Natural and Applied Sciences, Fountain University Osogbo, Osun State, Nigeria
2Industrial and Environmental Chemistry Unit, Department of Chemical Sciences, College of Natural and Applied Sciences, Fountain University Osogbo, Osun State, Nigeria
*Corresponding author
Abstract:

Extraction of Syzygium aromaticum powder using organic solvent (methanol) revealed the existence of alkaloids, flavonoids, terpenoids, tannins, aldehydes, ketones, alkaloids, glycoside, steroids, carbohydrates and phenolic compounds. A total of forty-six phenolic compounds were identified and quantified in Syzygium aromaticum extract using GCFID among which are gallic acid (847.36), syringic acid (259.04), protocatechuic acid (252.29), caffeic acid (151.01), eugenin (121.30), eugenitin (101.29), P-hydroxybenzoic acid (85.04), salicyclic acid (31.84), kaempferol (30.75), quercetin (27.68), rhamnetin (21.61), phenylacetic acid (18.09), myricetin (16.67) and isohamnetin (5.07) being of significant values. The percentage yield of Syzygium aromaticum oil extracted by hydrodistillation technique and isolated with Dichloromethane was 21.20%. The major phytoconstituents present are eugenol (75.10%), eugenyl acetate (13.57%), β-caryophyllene (5.27%), limonene (1.45%) and α-terpinolene (1.12%) respectively. The antimicrobial activity (minimum inhibitory concentration and minimum bactericidal concentration) of Syzygium aromaticum oil was investigated against six microorganisms namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus flavus and Penicillium spp using agar well diffusion and nutrient broth dilution techniques. Syzygium aromaticum oil showed excellent antimicrobial activity compared to ciprofloxacin and ketoconazole used as control by inhibiting both bacteria and fungi with wide zones of inhibition.


Keywords: Syzygium aromaticum, Gallic acid, Flavonoids, Eugenol, Eugenyl acetate
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How to cite this article:

Simiat Olanike Jimoh, Lateefah Adelanke Arowolo and Kazeem Adelani Alabi. 2017. Phytochemical Screening and Antimicrobial Evaluation of Syzygium aromaticum Extract and Essential oil.Int.J.Curr.Microbiol.App.Sci. 6(7): 4557-4567. doi: https://doi.org/10.20546/ijcmas.2017.607.476