Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 442-451
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.052


Isolation, Screening, Identification and Optimized Production of Extracellular Cellulase from Bacillus subtilis Sub.sps using Cellulosic Waste as Carbon Source
K.Venkateswar Reddy1*, T.Vijaya Lakshmi1, A.Vamshi Krishna Reddy1, V. Hima Bindu1 and M.Lakshmi Narasu2
1Center for Environment, Institute of Science and Technology, JNTUH, Kukatpally, Hyderabad-500085, Telangana, India
2Center for Biotechnology, Institute of Science and Technology, JNTUH, Kukatpally, Hyderabad-500085, Telangana, India
*Corresponding author
Abstract:

The bioconversion of cellulose is important for global stabilization and a suitable human society. In this study, efficient cellulolytic bacteria were screened and isolated from paper industry effluent contaminated soil. Among the isolates, three strains showing higher potential for cellulase production were purified and sub cultured on carboxy methyl cellulose (CMC) agar plates. Among the three strains, one strain was given maximum enzyme activity. The isolate were identified as Bacillus subtilis sub.sps by morphological, physiological, biological and 16S rRNA gene sequence analysis. The isolated strain produced cellulase enzyme complex, with suggested synergic cellulolytic systems in Bacillus subtilis sub.sps. Cellulase was produced in cellulase enzyme production media containing waste paper as carbon source and the culture conditions like temperature, pH, incubation time and medium components and nitrogen sources were optimized. Optimal concentration of inoculum for enzyme production is 2% and incubation time is 60hrs. Optimum temperature and pH of the medium for the cellulase production by Bacillus subtilis sub.sps was 45°C and pH 6-7.


Keywords: Cellulolytic bacteria,molecular identification, 16S rRNA, Bacillus subtilis sub.sps, cellulase
Download this article as Download

How to cite this article:

Venkateswar Reddy, K., T.Vijaya Lakshmi, A.Vamshi Krishna Reddy, V.Hima bindu, Lakshmi Narasu, M. 2016. Isolation, Screening, Identification and Optimized Production of Extracellular Cellulase from Bacillus subtilis Sub.sps using Cellulosic Waste as Carbon SourceInt.J.Curr.Microbiol.App.Sci. 5(4): 442-451. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.052