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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4412-4422
DOI: https://doi.org/10.20546/ijcmas.2017.607.460


Screening and Identification of Microorganisms for Enhanced Bioethanol Production from Pond Reeds
Vidhya Basak* and J.E. Nagamani
Department of Biotechnology, Garden City University, Bangalore, Karnataka, India
*Corresponding author
Abstract:

Phragmites australis, common pond reed with high lignocelluloses content is suitable for bioethanol production. In the present study pretreated pond reed was first fermented to sugars using cellulose and xylan degrading microorganisms isolated from cow dung and organic kitchen waste. Further fermentation of sugars to ethanol was performed using combinations of Sachharomyces cerevisae, Trichoderma reesei and Pichia stipitis. The isolated culture, O2.1 along with the combination of Trichoderma ressei and Pichia Stipitis exhibited maximum yield of ethanol (3.53gm %). Further identification of O2.1 was done by 16S-rDNA sequencing. The deduced sequence was used to retrieve the homologous sequences and to construct a phylogenetic tree. The results suggest the use of O2.1 for better ethanol production from pond reeds which can also help in value addition to the waste.


Keywords: Phragmites australis, Lignino cellulose, Fermentation, Bioethanol, Phylogenetic tree.
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How to cite this article:

Vidhya Basak and Nagamani, J.E. 2017. Screening and Identification of Microorganisms for Enhanced Bioethanol Production from Pond Reeds.Int.J.Curr.Microbiol.App.Sci. 6(7): 4412-4422. doi: https://doi.org/10.20546/ijcmas.2017.607.460