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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4310-4314
DOI: https://doi.org/10.20546/ijcmas.2017.607.448


Diagnostic Value of Sputum Gram’s Stain and Sputum Culture in Lower Respiratory Tract Infections in a Tertiary Care Hospital
Akansha Rana1*, Archana Sharma1 and Gaganpriya Pandey2
 1Department of Microbiology, WCMS&R, Jhajjar, Haryana, India
2Department of Microbiology, NIMS (Paramedical College), Jaipur, Rajasthan, India
*Corresponding author
Abstract:

There has always been a controversy in bacteriological assessment of sputum samples in diagnosing lower respiratory tract infections (LRTIs). Commonly in the Microbiological laboratory, expectorated sputum samples are microscopically examined for diagnosing LRTIs. The study was aimed to determine the diagnostic value of sputum gram’s stain and sputum culture in Lower Respiratory Tract Infections in a tertiary care hospital. Lower respiratory tract secretion (sputum) of 233 patients was cultured, identified and antimicrobial susceptibility by Kirby-Bauer disc-diffusion method was performed by standard methods. Quality of expectorated sputum samples were assessed by using Bartlett’s grading system. Among acceptable category, 141(77.05%) samples showed culture positivity. Among non-acceptable category, 42(22.92%) samples showed culture positivity. Streptococcus pneumoniae 37(20.22%) was the commonest isolated organism followed by Klebsiella pnemoniae-30(16.39%), Escherichia coli-22(12.03%), Staphylococcus aureus-21(11.47%) and Pseudomonas aeruginosa-18(09.84%). In this study, authors have recommended receiving good quality of sputum and the subsequent Gram stain and culture of sputum can provide a high diagnostic yield for clinically relevant LRTIs.


Keywords: Gram’s stain,Sputum culture,Lower respiratory tract infections, Bartlett’s criteria, Streptococcus pneumoniae, Klebsiella pneumoniae.
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How to cite this article:

Akansha Rana, Archana Sharma and Gaganpriya Pandey. 2017. Diagnostic Value of Sputum Gram’s Stain and Sputum Culture in Lower Respiratory Tract Infections in a Tertiary Care HospitalInt.J.Curr.Microbiol.App.Sci. 6(7): 4310-4314. doi: https://doi.org/10.20546/ijcmas.2017.607.448