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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(4): 413-423
DOI: http://dx.doi.org/10.20546/ijcmas.2016.504.049


Screening and Isolation of PHB Producers from Plant Sources
Aseem Rajan Wagle, Yogini Manohar Dixit and Babu Vamanrao Vakil*
Guru Nanak Institute for Research and Development, Guru Nanak Khalsa College of Arts, Science and Commerce, Matunga, Mumbai 400019, India
*Corresponding author
Abstract:

Polyhydroxybutyrate (PHB), a product of microbial metabolism, is the most commonly known biodegradable polymer that has emerged as an alternative to conventional synthetic and non biodegradable plastic. Traditionally PHB producers have been isolated from various sources like soil, marine environment, sewage, sludge, industrial effluent, etc.  The present study was aimed at isolating PHB producing bacteria from varied plant based sources. Using Nile Blue A as a primary qualitative screening method, 11 probable PHB positive bacteria were isolated and then evaluated for their PHB producing ability employing a conventional analytical method based on UV-spectrophotometric quantitation. The yields obtained were between 63-172 mg /L. Further characterization of PHB production was done using a less explored but powerful technique of flow cytometry. The flow cytometry revealed considerable variation in the PHB biosynthesis in the selected time range by the different isolates. The two different screening tools used to assess PHB accumulation confirmed the PHB producing ability of the isolates. The work presented shows that plant sources can be novel and promising sources for PHB isolation and hence should be explored more in future for isolation of diverse microflora.


Keywords: PHB, Plants,Nile Blue A,UV Spectrophoto-metry,Flow Cytometry
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How to cite this article:

Aseem Rajan Wagle, Yogini Manohar Dixit and Babu Vamanrao Vakil. 2016. Screening and Isolation of PHB Producers from Plant Sources.Int.J.Curr.Microbiol.App.Sci. 5(4): 413-423. doi: http://dx.doi.org/10.20546/ijcmas.2016.504.049