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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4231-4238
DOI: https://doi.org/10.20546/ijcmas.2017.607.438


Effect of Ethidium Bromide (EB) and N-methyl-N’-Nitro-N-Nitrosoguanidine (NTG) on Metabolic Activities of Streptomyces Strain N-404
Ismail Saadoun1* and Hazem Haddad2
1Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah, UAE
2Princes Haya Center for Biotechnology, Jordan University of Science and Technology,Irbid- 22110, Jordan
*Corresponding author
Abstract:

In this report, an attempt to study the effect of production of inhibitory bioactive compounds and fiber hydrolytic enzymes was demonstrated by mutagenic agent treatment analysis. The effects of two chemical mutagenic agents [N-methyl-N'-nitro-N-nitrosoguanidine (NTG) (65-500 µg/ml) and Ethidium Bromide (EB) (5-25 µM)] on the loss of enzymes and antibiotic activities and generation of bald mutants (no aerial mycelia) in Streptomyces strain N-404, were observed. Generation of bald mutants was observed with treatment with 65 µg/ml of NTG and 10 µM EB after 5 and 14 days, respectively. Production of antibiotics and fiber hydrolytic enzymes (amylase, cellulase, and xylanase) was not eliminated, but noticeable reduction of these activities in the mutant strain was detected.


Keywords: Aerial mycelium, bald mutant, Antibiotic, Enzyme, Streptomyces
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How to cite this article:

Ismail Saadoun and Hazem Haddad. 2017. Effect of Ethidium Bromide (EB) and N-methyl-N’-Nitro-N-Nitrosoguanidine (NTG) on Metabolic Activities of Streptomyces Strain N-404.Int.J.Curr.Microbiol.App.Sci. 6(7): 4231-4238. doi: https://doi.org/10.20546/ijcmas.2017.607.438