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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4219-4230
DOI: https://doi.org/10.20546/ijcmas.2017.607.437


Assessing Genetic Diversity of Potato (Solanum tuberosum) Genotypes Grown in Tarai Region of Uttarakhand by Using Simple Sequence Repeat (SSR) Technique
Rajani*, Dhirendra Singh and A.S. Jeena
Department of Vegetable Science, G. B. Pant University of Agriculture and Technology, India
*Corresponding author
Abstract:

This study aimed to evaluate the genetic diversity and identify potato cultivars by SSR markers. The genomic DNA of 50 potato cultivars was amplified with nineteen Simple Sequence Repeats (SSRs) primers. Out of nineteen primers only fifteen primers were amplified and gave polymorphic bands. Therefore, fifteen SSR primers were used in diversity analysis of 50 potato genotypes. Out of fifteen primers, four primers were found to be monomorphic and rests were polymorphic. 11 SSR primers pairs which were amplified polymorphic band that generated 34 polymorphic bands. Most of the primers detected more than one loci and observed to be polymorphic thus able to differentiate different genotypes. The dendrograms generated by cluster analysis distinguished the cultivars genetically. The PIC values demonstrated the high information content of the primers used and 50 potato varieties were identified based on SSR primer pairs. Thus, by means SSR markers the genetic diversity was assessed and the 50 potato genotypes analyzed in this study were identified.


Keywords: Solanum tuberosum,Micro-satellites, Genetic diversity.
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How to cite this article:

Rajani, Dhirendra Singh and Jeena, A.S. 2017. Assessing Genetic Diversity of Potato (Solanum tuberosum) Genotypes Grown in Tarai Region of Uttarakhand by Using Simple Sequence Repeat (SSR) TechniqueInt.J.Curr.Microbiol.App.Sci. 6(7): 4219-4230. doi: https://doi.org/10.20546/ijcmas.2017.607.437