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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4201-4209
DOI: https://doi.org/10.20546/ijcmas.2017.607.435


Expression of Defense Responsive Gene in Rice after the Infection of Rhizoctonia solani
Raja Husain1, N.A. Khan1, A. Mishra2, M.K. Sharma2, A. Singh2, R.S. Sengar2,M.K. Yadav2, Vinay K. Yadav1, Jaygendra Kumar1, Harishchandra, K. Gyanendra1, Shivani1, N. Vikram3, and K.N. Singh1*
1Department of Plant Molecular Biology and Genetic Engineering, Narendra Deva University of Agriculture and Technology Kumarganj Faizabad (U.P.) India
2Department of Agriculture Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.) India
3Department of Biochemistry, Zila Parishad Agriculture College, Banda (U.P.) India
*Corresponding author
Abstract:

Rice sheath blight, caused by Rhizoctonia solani is one of the major diseases of rice. The pathogen infects rice plants directly through stomata or using lobate appressoria and hyphal masses called infection cushions. Initially the mycelium looked thread like structure, milky white in colour than mycelium converted mature Sclerotia.  After the screening of different rice genotypes using 0 to 9 scaling (SES-IRRI), according to Leaf detach method, Pusa Basmati-1 was found highly susceptible variety and Swarna sub-1 was susceptible one. Among wild rice, O. australiensis was observed resistant species, while Oryza rufipogon was found moderately resistant species. Approximately the same results were found in humidified chamber condition. During molecular analysis, In control condition, 8A1 gene/primer was not expressed in Pusa Basmati-1 and Swarna sub-1, it was expressed in stressed condition in both the cultivar with the product size 155 bp. In both wild rice namely O. rufipogon and O. australiensis, 8A1 gene was expressed in control as well as stressed condition with the product size 155 bp. While 4C7 gene/primer was expressed continuously in control and stressed condition in susceptible rice cultivars; Pusa Basmati-1, Swarna sub-1 and wild rice accessions- O. rofipogon and O. australiensis with the product size 162 bp.


Keywords: Sheath blight, SES, 8 A1, 4C7, R. solani.
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How to cite this article:

Raja Husain, N.A. Khan, A. Mishra, M.K. Sharma, A. Singh, R.S. Sengar, M.K. Yadav, Vinay K. Yadav, Jaygendra Kumar, Harishchandra, K. Gyanendra, Shivani, N. Vikram, and Singh, K.N. 2017. Expression of Defense Responsive Gene in Rice after the Infection of Rhizoctonia solani.Int.J.Curr.Microbiol.App.Sci. 6(7): 4201-4209. doi: https://doi.org/10.20546/ijcmas.2017.607.435