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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 4139-4147
DOI: https://doi.org/10.20546/ijcmas.2017.607.429


Genetic Diversity Analysis of Labeo rohita (Hamilton, 1822) Collected from Two Zones of River Ganga Using Microsatellite DNA Markers
Deepak Joshi1*, Pushpa Lohni2 and R.N. Ram1
1Department of Fisheries Resource Management, College of Fisheries, G. B. Pant University of Agriculture and Technology, Pantnagar, India
2Department of Molecular Biology and Generic Engineering, College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, India
*Corresponding author
Abstract:

The present study was conducted to investigate the level of polymorphism and genetic diversity in natural population of Labeo rohita collected from selected zones of River Ganga using microsatellite DNA markers. 5 primers out of 10 produced amplicons and were selected for primary screening and further genetic analysis of the population. On the basis of the values of Average Nei’s Genetic Diversity, observed and expected heterozygosity, fixation index, Shannon’s information index obtained through microsatellite marker technique, the present study is the indicative of the fact that the stock of L. rohita of Haridwar region of River Ganga is more genetically diverse and genetically differentiated as compared to the stock of Kanpur region as these sites are distantly located and having negligible chance of exchange of gene pool with each other which is responsible for weak sub-structuring of stocks. Genetic variation observed between the two riverine sites may also be caused due to high level of anthropogenic activities in Kanpur region thereby causing blockage of fish movement as well destruction of breeding grounds thus hampering the proper mating within the population and also reducing effective population size.


Keywords: Labeo rohita, River Ganga, Microsatellite markers, Genetic diversity.
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How to cite this article:

Deepak Joshi, Pushpa Lohni and Ram, R.N. 2017. Genetic Diversity Analysis of Labeo rohita (Hamilton, 1822) Collected from Two Zones of River Ganga Using Microsatellite DNA Markers.Int.J.Curr.Microbiol.App.Sci. 6(7): 4139-4147. doi: https://doi.org/10.20546/ijcmas.2017.607.429