National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Detection of Methicillin resistant strains of Staphylococcus aureus using phenotypic and genotypic methods in a tertiary care hospital Institution: Institute of Microbiology, Madras Medical College. Abstract: Purpose: Methicillin resistant S. aureus (MRSA) are significant pathogens that have emerged over the past 30 years to cause both nosocomial and community acquired infections. This study has been undertaken to compare the conventional and molecular methods in detecting MRSA. S. aureus was isolated from pus samples and identified by Gram stain and Catalase test. Further confirmation was done by slide and tube coagulase test, growth on MSA, DNA se test. Methicillin resistance was determined by disc diffusion method, oxacillin screen agar, micro broth dilution test and also by detection of mecA gene by multiplex PCR method. The antimicrobial susceptibility pattern of the isolates was determined by Kirby-Bauer disc diffusion method. Vancomycin resistance was determined by disc diffusion and E-test method as per CLSI guidelines. Results: A total of 150 S. aureus was isolated from pus samples. Phenotypic methods detected 54% of isolates as methicillin resistant and 46% as methicillin susceptible. Detection of mecA gene was done by multiplex PCR in 50 isolates which showed 29 isolates (58%) as methicillin resistant. All the isolates were sensitive to Vancomycin by disc diffusion method and E test. Antibiotic sensitivity results showed that MRSA strains were more resistant to antibiotics compared to methicillin sensitive Staphylococcus aureus (MSSA) isolates. Conclusion: The conventional phenotypic methods were comparable with that of mecA gene detection, the gold standard and hence it can be used in the detection of MRSA isolates.
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