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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2769-2781
DOI: https://doi.org/10.20546/ijcmas.2017.607.387


Production and Improvement of Alkaline α - Amylase by Bacterial Isolate from Chilika Lake
Allampalli Sirisha, Sonali Priyadarshini* and Pratima Ray
Department of microbiology, C.B.S.H., Orissa University of Agriculture and Technology, Unit 7, Surya Nagar, Bhubaneshwar, Odisha-751003, India
*Corresponding author
Abstract:

The present study suggest that the Chilika Lake isolate i.e., Bacillus sp. may act as a potent strain for alkaline α- amylase production. In case of parent strain the enzyme production was higher with banana peels as the substrate; in case of mutant, potato peels used as substrate. Optimization of the fermentation parameters and the use of suitable carbon, nitrogen and metal chloride supplements resulted in increase in the enzyme yield. The enzyme production was significantly high at optimum temperature which was found to be 37 °C and the optimum inoculums size 2%. Maximum production was found when the medium is supplemented with starch as carbon source, yeast extract as nitrogen source and NaCl as mineral chloride. The enzyme was found to be active over a wide range of pH and showed the optimum activity at pH 11. This isolate both wild and mutant could thus be industrially exploited for the synthesis of alkaline α- amylase which have immense industrial application.


Keywords: Solid state fermentation, Bacillus sp, Alkaline α- amylase, UV induced mutation, Sediment sample.
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How to cite this article:

Allampalli Sirisha, Sonali Priyadarshini and Pratima Ray. 2017. Production and Improvement of Alkaline α - Amylase by Bacterial Isolate from Chilika Lake.Int.J.Curr.Microbiol.App.Sci. 6(7): 2769-2781. doi: https://doi.org/10.20546/ijcmas.2017.607.387