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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(7): 2688-2695
DOI: https://doi.org/10.20546/ijcmas.2017.607.378


Phytochemical Profiling, Antibacterial Potential and GC-MS Analysis of Methanol Leaf Extract of Enicostemma axillare
K.Mullai Nila and J. Karthikeyan*
Department of Zoology, Presidency College (Autonomous), Chennai-600005, Tamil Nadu, India
*Corresponding author
Abstract:

Aim of the study is to identify and characterize the bioactive compounds from methanol leaf extract of Enicostemma axillare responsible for the bactericidal activity. The in vitro antibacterial activity was determined by disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterococcus faecalis and Bacillus subtilis. Gas Chromatography-Mass Spectrometry (GC-MS) studies were carried out to identify the presence of bioactive compounds. Antibacterial studies reveal a dose-dependent zone of inhibition. A maximum zone of inhibition was observed with reference to Klebsiella pneumoniae (12mm) followed by Escherichia coli (10mm). Observation of GC-MS results shows the presence of antibacterial compounds glycine, N- methyl ester, octadecatrienoic acid, 1-heptatriacetanol, and tetramethyl-2-hexadecen-1-ol were identified. It is concluded that the methanolic leaf extract from Enicostemma axillare possesses the potential compounds responsible for the antibacterial activity.


Keywords: Enicostemma axillare, antibacterial activity, E. coli, GC-MS, Phytol.
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How to cite this article:

Mullai Nila, K. and Karthikeyan, J. 2017. Phytochemical Profiling, Antibacterial Potential and GC-MS Analysis of Methanol Leaf Extract of Enicostemma axillare.Int.J.Curr.Microbiol.App.Sci. 6(7): 2688-2695. doi: https://doi.org/10.20546/ijcmas.2017.607.378